Pharmacokinetics of a Chinese Traditional Medicine, Denshensu (3,4-Dihydroxyphenyllactic Acid), in Rabbits Using High-Performance Liquid Chromatography

概要

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An injectable solution of Danshen was prepared and its in vivo disposition was examined in rabbits. The presence of Danshensu, one of the active components of Danshen, in the obtained solution was confirmed by a simple high-performance liquid chromatographic (HPLC) method. The pharmacokinetics of Danshensu in rabbits was evaluated by the HPLC method for plasma Danshensu. The calibration curve for Danshensu was linear (r=0.998) over the concentration range of 0.25-40.0 μg/ml. The intra-assay coefficients of variation (CV_s) were 3.8,3.1,and 3.1% at 1,10,and 50 μg/ml, respectively, and the inter-assay CV_s were 5.3,5.3,and 2.9% at 1,10,and 50 μg/ml, respectively. The analytical recovery of Danshensu in plasma averaged 95.2%. From the plasma concentraion profile of Danshensu after its intravenous administration, the t_<1/2>, mean residence time (MRT), V_<dss>, and Cl<tot> were determined as 32 min, 48 min, 149 ml/kg, and 3.13 ml/min/kg, respectively.
公益社団法人日本薬学会の論文
1997-03-15