Inhibitory Effect of Sanguiin H-11 on Chemotaxis of Neutrophil

概要

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Neutrophil is accumulated by chemoattractants at inflammatory sites. In order to find a new substance to regulate the chemotaxis of neutrophil, we examined the effects of purified tannins and related compounds (33 species). Among those studied, sanguiin H-11,purified from the plant Sanguisorba officinalis, was the most potent inhibitor of rat cytokine induced neutrophil chemoattractant-1 (CINC-1,a counterpart of human GRO(melanoma growth-stimulating activity)) dependent chemotaxis in rat neutrophils. Sanguiin H-11 inhibited platelet-activating factor- and N-formylmethionyl-leucyl-phenylalanine-dependent chemotaxis of neutrophil in addition to CINC-1-dependent reactions. Sanguiin H-11 also inhibited chemokinesis of neutrophil, but did not drastically affect the increase of intracellular free [Ca^<2+>] level or degranulation monitored by the secretion of elastase, following chemoattractant stimulation. These results indicated that sanguiin H-11 is a potent inhibitor of chemoattractant-dependent and independent neutrophil movement.
公益社団法人日本薬学会の論文
2000-02-01