Nicotinic Enhancement of Proliferation in Bovine and Porcine Cerebral Microvascular Endothelial Cells(Pharmacology)
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概要
- 論文の詳細を見る
Nicotinic acetylcholine receptors are found in microvascular endothelial cells. To reveal the functional role in cerebral angiogenic processes, we studied the nicotinic modulation of proliferation activity in cultured bovine and porcine cerebral microvascular endothelial cells. The proliferation activity was determined by an increase in the number of cells present in culture dishes. When the bovine cerebral endothelial cells at different passages were cultured in the presence of nicotine (10 nM), the proliferation activities were significantly increased in the cells at passage 1 and passage 3, but not at passage 4. Reverse transcriptase-polymerase chain reaction studies demonstrated that the expression of mRNAs coding for α3 nicotinic receptor subunit was significantly reduced in the bovine cerebral endothelial cells at passage 4, compared with that at passage 1. The proliferation of porcine cerebral endothelial cells (passage 1) was enhanced by acetylcholine (10 nM-100 μM) in the presence of atropine, a muscarinic antagonist, and this enhancing effect was inhibited by hexamethonium (100μM, a nicotinic antagonist). The stimulation by acetylcholine (1 μM, with atropine) or nicotine (10 nM) induced the phosphorylation of a mitogen-activated protein (MAP) kinase (extracellular-signal regulated kinase : ERK) in the serum-starved endothelial cells. In the presence of PD98059 (2 μM, a MAP kinase kinase inhibitor) and atropine, acetylcholine (1 μM) failed to enhance the proliferation of porcine cerebral endothelial cells. These results demonstrate that nicotinic stimulation promotes the proliferation of bovine and porcine cerebral microvascular endothelial cells, at least in part, through the MAP kinase activation.
- 公益社団法人日本薬学会の論文
- 2004-12-01
著者
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木村 郁子
富山医薬大薬薬品作用学
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木村 郁子
富山医科薬科大学
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木村 郁子
富山医科薬科大学 臨床薬理学
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木村 郁子
富山医科薬科大学大学院薬学研究科臨床薬理学
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木村 郁子
富山医科薬科大学 大学院薬学研究科臨床薬理学講座
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KIMURA Ikuko
Department of Chemical Pharmacology Faculty of Pharmaceutical Sciences, Toyama medical and Pharmaceu
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KAWAKAMI Junichi
Department of Pharmacy, The University of Tokyo Hospital, Faculty of Medicine, University of Tokyo
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TSUNEKI Hiroshi
Department of Clinical Pharmacology, Toyama Medical and Pharmaceutical University
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Inaba Kazuhiko
Department Of Chemical Pharmacology Faculty Of Pharmaceutical Sciences Toyama Medical And Pharmaceut
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Inaba Kazuhiko
北陸大学 薬 薬理
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Ito Kana
Department Of Clinical Pharmacology Toyama Medical And Pharmaceutical University
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Kimura I
Toyama Medical And Pharmaceutical Univ. Toyama Jpn
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Kimura Ikuko
Department Of Chemical Pharmacology Faculty Of Phrmaceutical Sciemes Toyama Medical And Pharmaceutic
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Kimura I
Department Of Ophthalmology Keio University School Of Medicine
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木村 郁子
富山医科薬科大学 薬学部 薬品作用学
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Kimura Ikuko
Department Of Chemical Pharmacology Faculty Of Pharmaceutical Sciences Toyama Medical And Pharmaceut
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ADACHI Isao
Department of Hospital Pharmacy, Toyama Medical and Pharmaceutical University
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Tsuneki H
Department Of Clinical Pharmacology University Of Toyama
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Sasaoka Toshiyasu
Department of Clinical Pharmacology, University of Toyama
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YOU Yueren
Department of Clinical Pharmacology, Toyama Medical and Pharmaceutical University
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SEKIZAKI Naoto
Department of Clinical Pharmacology, Toyama Medical and Pharmaceutical University
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MA En-Long
Department of Clinical Pharmacology, Toyama Medical and Pharmaceutical University
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Ma En-long
Department Of Clinical Pharmacology Toyama Medical And Pharmaceutical University
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You Yueren
Department Of Clinical Pharmacology Toyama Medical And Pharmaceutical University
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Sekizaki Naoto
Department Of Clinical Pharmacology Toyama Medical And Pharmaceutical University
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Adachi Isao
Department Of Hospital Pharmacy Toyama Medical And Pharmaceutical University
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木村 郁子
Department Of Clinical Pharmacology Graduate School Of Pharmaceutical Sciences Toyama Medical And Ph
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Tsuneki Hiroshi
Department Of Chemical Pharmacology Faculty Of Pharmaceutical Sciences Toyama Medical And Pharmaceut
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Tsuneki Hiroshi
富山医科薬科大学大学院薬学研究科 臨床薬理学講座
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Sasaoka Toshiyasu
Department Of Clinical Pharmacology University Of Toyama
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Sasaoka Toshiyasu
Department Of Clinical Pharmacology Toyama Medical & Pharmaceutical University First Department
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Kawakami Junichi
Department Of Hospital Pharmacy Hamamatsu University School Of Medicine
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Kimura Ikuko
Department Of Chemical Pharmacology Faculty Of Pharmaceutical Sciences Toyama Medical & Pharmace
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Kimura Ikuko
Deparment Of Chemical Pharmacology Faculty Of Pharmaceutical Sciences Toyama Medical And Pharmaceutical University
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木村 郁子
Department of Food and Nutrition Science, Toyama College
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木村 郁子
富山医科薬科大学大学院薬学研究科 臨床薬理学講座
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ADACHI Isao
Department of Applied Mathematics Osaka University
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TSUNEKI Hiroshi
Department of Clinical Pharmacology,Graduate School of Pharmaceutical Sciences,Toyama Medical and Pharmaceutical
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