A Quantitative Assay for Angiogenesis of Cultured Choroidal Tissues in Streptozotocin-Diabetic Wistar and Spontaneously Diabetic GK Rats
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概要
- 論文の詳細を見る
Angiogenesis of cultured choroids was quantitatively assayed in spontaneously diabetic GK and a bolus-treated streptozotocin (STZ)-diabetic Wistar rats. The number and total length of microvessels budded from cultured choroidal explants were measured to use as angiogenic indices. Both indices in 10-week-old Wistar rats were increased in parallel by 5% fetal bovine serum (FBS) from days 2 to 7 in culture. These indices in STZ-rats (10 weeks of age) were increased by 5% FBS to a greater extent than those in age-matched normal rats. These enhanced actions of FBS were concentration-dependent. The explants of 16-week-old GK rats also increased these indices to a greater extent than those of age-matched Wistar rats. Aging to 18 weeks of age also increased choroidal angiogenesis in the normal rats. In conclusion, the assay model of choroidal angiogenesis was established by determining the number and length of microvessels in cultured choroidal explants. The diabetic states of STZ-Wistar and GK rats enhanced FBS-induced choroidal angiogenesis. This assay model is useful for determining angiogenic activity of growth factors and effective drugs in diabetic choroidopathy and retinopathy.
- 社団法人 日本薬理学会の論文
- 1998-12-01
著者
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木村 郁子
富山医科薬科大学
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木村 郁子
富山医科薬科大学大学院薬学研究科臨床薬理学
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木村 郁子
富山医科薬科大学 大学院薬学研究科臨床薬理学講座
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KIMURA Ikuko
Department of Chemical Pharmacology Faculty of Pharmaceutical Sciences, Toyama medical and Pharmaceu
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KOBAYASHI Shinjiro
Department of Pharmacology, Faculty of Pharmaceutical Sciences, Hokuriku University
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FUKUTA Mizuki
Department of Pharmacology, Faculty of Pharmaceutical Sciences, Hokuriku University
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KONTANI Hitoshi
Department of Pharmacology, Faculty of Pharmaceutical Sciences, Hokuriku University
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Fukuta Mizuki
Department Of Pharmacology Faculty Of Pharmaceutical Sciences Hokuriku University
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Fukuta Mizuki
北陸大学 薬 薬理
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Inaba Kazuhiko
Department Of Chemical Pharmacology Faculty Of Pharmaceutical Sciences Toyama Medical And Pharmaceut
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Inaba Kazuhiko
北陸大学 薬 薬理
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Kontani H
Department Of Pharmacology Faculty Of Pharmaceutical Sciences Hokuriku University
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Kontani H
Hokuriku Univ. Kanazawa Jpn
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Kimura I
Toyama Medical And Pharmaceutical Univ. Toyama Jpn
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Kimura Ikuko
Department Of Chemical Pharmacology Faculty Of Phrmaceutical Sciemes Toyama Medical And Pharmaceutic
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Kimura I
Department Of Ophthalmology Keio University School Of Medicine
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Kimura Ikuko
Department Of Chemical Pharmacology Faculty Of Pharmaceutical Sciences Toyama Medical And Pharmaceut
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YANAGITA Sumino
Department of Chemical Pharmacology, Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmace
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Kobayashi Shinjiro
Department Of Chemical Pharmacology Toyama Medical And Pharmaceutical University
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Kontani Hitoshi
Department Of Pharmacology Faculty Of Pharmaceutical Science Hokuriku University
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Kobayashi S
Kyoritsu Coll. Pharmacy
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木村 郁子
Department Of Clinical Pharmacology Graduate School Of Pharmaceutical Sciences Toyama Medical And Ph
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Yanagita Sumino
Department Of Chemical Pharmacology Faculty Of Pharmaceutical Sciences Toyama Medical And Pharmaceut
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Kimura Ikuko
Department Of Chemical Pharmacology Faculty Of Pharmaceutical Sciences Toyama Medical & Pharmace
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Kobayashi Shinjiro
Department Of Clinical Pharmacology Faculty Of Pharmaceutical Sciences Hokuriku University:organizat
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Fujihara Mitsuhiro
Department Of Chemical Pharmacology Faculty Of Pharmaceutical Sciences Toyama Medical And Pharmaceut
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Kimura Ikuko
Deparment Of Chemical Pharmacology Faculty Of Pharmaceutical Sciences Toyama Medical And Pharmaceutical University
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木村 郁子
Department of Food and Nutrition Science, Toyama College
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木村 郁子
富山医科薬科大学大学院薬学研究科 臨床薬理学講座
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KONTANI Hitoshi
Department of Chemical Pharmacology, Faculty of Pharmaceutical Sciences, Nagoya City University
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Kontani Hitoshi
Departmenr of Pharmacology, Faculty of Pharmaceutical Science, Hokuriku University
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Kobayashi Shinjiro
Department of Applied Chemistry, Faculty of Engineering, Kyushu University
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