Protein Kinase Activity Associated with the IME2 Gene Product, a Meiotic Inducer in the Yeast Saccharomyces cerevisiae
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概要
- 論文の詳細を見る
The IME2 gene product (Ime2) is required for entry into meiosis and sporulation in S. cerevisiae. It has been predicted to be composed of two domains, an amino-terminal domain with homology to protein kinases and a carboxy-terminal acidic domain. The Ime2 was identified in extracts of meiotic cells carrying multi- but not low-copy IME2 in immunoblot analysis using an Ime2-specific antibody. Immune complexes were found to phosphorylate Ime2 and several exogenous proteins. Low-copy plasmids expressing truncated Ime2 proteins that lack part of or the entire carboxy-terminal domain enabled cells to undergo sporulation even under a certain repressive nutritional condition. These cells contained increased levels of protein kinase activity compared with control cells. These results suggest that the amino-terminal domain has a protein kinase activity and that the acidic tail is not essential for either the kinase activity or sporulation but serves in a negative role. An Ime2-beta-galactosidase fusion was shown by immunofluorescence microscopy to be localized predominantly to the nucleus, suggesting a nuclear function of Ime2.
- 社団法人日本農芸化学会の論文
- 1993-10-23
著者
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YAMASHITA Ichiro
Center for Gene Science, Hiroshima University
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Yamashita Ichiro
Center For Gene Science Hiroshima University
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Sakata Yushi
Faculty Of Agriculture Kagoshima University
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Kominami Kin-ichiro
Center for Gene Science, Hiroshima University
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Sakata Yushi
Center for Gene Science, Hiroshima University
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Sakai Masami
Center for Gene Science, Hiroshima University
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Sakata Yushi
Center For Gene Science Hiroshima University
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Yamashita I
Hiroshima Univ. Higashihiroshima Jpn
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Sakai Masami
Center For Gene Science Hiroshima University
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Kominami Kin-ichiro
Center For Gene Science Hiroshima University
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