Fed-Batch Culture with a Modified DO-Stat Method
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概要
- 論文の詳細を見る
To support a high growth rate of microorganism in fed-batch culture with high cell dentisy, a modified DO-stat method was developed. In this method, as exponential substrate feed was coupled with the usual DO-stat method, i.e., a fixed amount of substrate per DO signal was exponentially fed to the culture based on the estimation of the substrate consumption rate and thereafter the feed was stopped in order to prevent the oversupply of substrate until an abrupt increase in the concentration of dissolved oxygen(DO)in the broth appeared. After that, the feed was started again and this cyclic operation was repeated throughout the cultivation. This method was applied to the fed-batch cultivation of ethanol utilizing yeast, Candida brassicae. At high cell densities(>10g/l), this modified method was more effective than the usual one in keeping a higher growth rate.
- 社団法人日本生物工学会の論文
- 1991-01-25
著者
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ENDO Takahiko
Department of Physics,Faculty of Science and Technology,Keio University
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Endo Takahiko
Department Of Fermentation Tachnology Faculty Of Engineering Hiroshima University
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YANO TAKUO
Department of Fermentation Tachnology, Faculty of Engineering, Hiroshima University
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TUJI TAKASHI
Department of Fermentation Tachnology, Faculty of Engineering, Hiroshima University
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NISHIZAWA YOSHINORI
Department of Fermentation Tachnology, Faculty of Engineering, Hiroshima University
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Nishizawa Y
Yamaguchi Univ. Ube Jpn
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Nishizawa Yoshinori
Department Of Applied Chemistry And Chemical Engineering Faculty Of Engineering Yamaguchi University
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Yano Takuo
Department Of Fermentation Tachnology Faculty Of Engineering Hiroshima University
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Tuji Takashi
Department Of Fermentation Tachnology Faculty Of Engineering Hiroshima University
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