Prediction of the Concentration of Several Constituents in a Mouse-Mouse Hybridoma Culture by Near Infrared Spectroscopy
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概要
- 論文の詳細を見る
Near infrared spectroscopy (NIR) was employed to predict the concentration of the nutrients, glucose and glutamine, and the products, lactic acid and ammonia, in the culture broth of a mouse-mouse hydridoma. A supernatant of the culture broth was supplied to a near infrared spectrophotometer and the absorbance at wavelengths between 1,100 and 2,500 mn was measured. The correlations between the concentrations obtained by conventional methods and obtained by NIR were examined by multiple regression analysis. NIR values predicted by 5- wavelength regression were in good agreement with those obtained by the usual methods. The multiple correlation coefficient was over 0.98 for all four substances. The procedure involved in NIR is simple, and the operation time required to predict the concentrations is only 5 min. The results suggest that NIR is a useful method for the control of animal cell cultures.
- 社団法人日本生物工学会の論文
- 1994-06-25
著者
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YANO TAKUO
Department of Fermentation Tachnology, Faculty of Engineering, Hiroshima University
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Yano Takuo
Department Of Fermentation Tachnology Faculty Of Engineering Hiroshima University
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HARATA MASAMI
Department of Fermentation Technology, Faculty of Engineering, Hiroshima University
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Harata Masami
Department Of Fermentation Technology Faculty Of Engineering Hiroshima University
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