Stabilization of Free and Immobilized Enzymes Using Hyperthermophilic Chaperonin(ENZYMOLOGY, PROTEIN ENGINERING, AND ENZYME TECHNOLOGY)

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概要

• 論文の詳細を見る

• Chaperonins suppress the denaturation of proteins and promote protein folding in vivo. Because hyperthermophilic chaperonins are expected to be used as a stabilizer for proteins, the effects of a group II chaperonin from a hyperthermophilic archaeum, Thermococcus strain KS-1 (T. KS-1 cpn), on the stabilization of mesophilic and thermophilic free enzymes and an enzyme co-immobilized with T. KS-1 cpn were studied. T. KS-1 cpn prevented the thermal inactivation of yeast alcohol dehydrogenase (ADH), jack bean urease, and Thermus flavus malate dehydrogenase (MDH) at high temperatures. T. KS-1 cpn also improved the long-term stability of ADH at lower temperatures. Moreover, the residual ADH activity of ADH co-entrapped with T. KS-1 cpn was improved and maintained at a higher level than that of the entrapped ADH without chaperonin. T. KS-1 cpn is useful for the stabilization of free and immobilized enzymes and applicable to various fields of biotechnology.

• 社団法人日本生物工学会の論文
• 2006-02-25

著者

• YANO TAKUO

  Department of Fermentation Tachnology, Faculty of Engineering, Hiroshima University

• Kohda J

  Hiroshima City University

• Kohda Jiro

  Division Of Molecular Science Graduate School Of Science And Technology Kobe University:(present Add

• Yano Takuo

  Hiroshima City University

• Yano Takuo

  Department Of Fermentation Tachnology Faculty Of Engineering Hiroshima University

• SUEHARA Ken-ichiro

  Department of Biotechnology, Graduate School of Engineering, Nagoya University

• Suehara Ken-ichiro

  Hiroshima City University

• Nakano Yasuhisa

  Hiroshima City University

• KOHDA Jiro

  Department of Information Machines and Interfaces, Faculty of Information Sciences, Hiroshima City U

• NAKANO Yasuhisa

  Department of Information Machines and Interfaces, Faculty of Information Sciences, Hiroshima City U

• Kawanishi Hirofumi

  Department of Information Machines and Interfaces, Faculty of Information Science, Hiroshima City Un

• Nakano Y

  Hiroshima City University

• Nakano Yasuhisa

  Department Of Information Machines And Interfaces Faculty Of Information Sciences Hiroshima City Uni

• Kawanishi Hirofumi

  Department Of Information Machines And Interfaces Faculty Of Information Science Hiroshima City Univ

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