Efficient Production of Celery Embryos and Plantlets Released in Culture of Immobilized Gel Beads

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概要

• 論文の詳細を見る

• Celery embryos and plantlets were found to be selectively released in a culture of immobilized Ca-alginate gel beads in which celery callus was entrapped under regeneration conditions. We studied the feasibility of use of this process for celery embryogenesis in an artificial seed system. The cells released from the gel beads were larger than those obtained in suspension culture. The optimal concentration of alginate gel for embryo and plantlet production was 2% for the immobilized cell culture. Considering the maintenance of the gel bead structure and detrimental effect of CaCl_2 on plantlet development, 5 mM CaCl_2 supplementation gave the best result in terms of the number of heart and torpedo embryos and plantlets. The ratio of the number of heart embryos, torpedo embryos and plantlets to total number of cells in the immobilized cell culture was higher than that in the suspension culture. Repeated batch culture with 5 mM CaCl_2 provided long-term(more than 154 d) embryo and plantlet production without gel beads disruption. Productivity of plantlets in the immobilized cell culture with 5 mM CaCl_2 was 2.2-fold as high as that in the suspension culture.

• 社団法人日本生物工学会の論文
• 1995-06-25

著者

• KOBAYASHI Takeshi

  Department of Electrical Engineering, Faculty of Engineering Science, Osaka University

• Uozumi N

  Nagoya Univ. Nagoya Jpn

• Kobayashi T

  Graduate School Of Marine Science And Technology Tokyo University Of Marine Science And Technology

• Kobayashi Takeshi

  Department Of Biological Chemistry College Of Bioscience And Biotechnology Chubu University

• UOZUMI NOBUYUKI

  Department of Biotechnology, Faculty of Engineering, Nagoya University

• Kobayashi T

  Dep. Of Biological Mechanisms And Functions Graduate School Of Bioagricultural Sciences Nagoya Univ.

• SUEHARA Ken-ichiro

  Department of Biotechnology, Graduate School of Engineering, Nagoya University

• KOHKETSU KATSUHITO

  Department of Biotechnology, Faculty of Engineering, Nagoya University

• Suehara Ken-ichiro

  Hiroshima City University

• Uozumi Nobuyuki

  Department Of Biomolecular Engineering Graduate School Of Engineering Tohoku University

• Kohketsu Katsuhito

  Department Of Biotechnology Faculty Of Engineering Nagoya University

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