Ethanol Production by Immobilized Cells with Forced Substrate Supply

概要

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Ethanol fermentation by a forced substrate supply into an immobilized cell layer was carried out to in crease the ethanol production rate and to eliminate the diffusion dependency of substrate supply in an ordinary immobilized cell reaction. Saccharomyces cerevisiae IFO 2347 was immobilized in a mixture of k_carrageenan, locust bean gum, and celite (2 : 0.5 : 40 wt/vol %). A glucose minimal medium was fed into the immobilized cell layer (5 to 22 mm in thickness) at retention times between 0.6 and 2.8 h under pressure. The stable ethanol fermentation could be maintained for more than 3 weeks with an ethanol yield of 0.48 g ethanol/g glucose and ethanol productivity of 63g・(l gel)^<-1>・h^<-1> at a retention time of 1.5 h. The yeast cells were well distributed through the gel layer with a vertival gradient, and an average cell density was ca. 8.0×10^9 cells/ml gel, 4-fold higher than that of ordinary immobilized cells. A small filter press reactor was constructed to examine the applicability of ethanol fermentation with this forced substrate supply. The option could be continued for a month at a retention time of 2 h yielding 96 g/l of ethanol from 200 g/l of glucose.
社団法人日本生物工学会の論文
1984-06-25