Limiting Factor of Nitrogenase System Mediating Hydrogen Production of Rhodobacter sphaeroides S
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概要
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To explain the decrease of hydrogen production rate in the batch culture of Rhodobacter sphaeroides S, the activities of enzymes related to the TCA cycle, nitrogenase in cell-free extracts, ATP generation by chromatophores, and ferredoxin were examined at the beginning, middle and end of the hydrogen production phase of batch culture. The activities of TCA cycle enzymes, nitrogenase and ATP generation were found to remain at almost the same level throughout the culture, while ferredoxin activity decreased linearly with time. In addition, by bubbling N_2 gas into the culture broth at the end of the culture, the hydrogen production rate was restored to the initial level through the increase of the ferredoxin activity. Although the decrease of ferredoxin activity and its restoration by bubbling N_2 gas remained unexplained, ferredoxin activity was considered to be a key function in the nitrogenase system for H_2 production by this photosynthetic bacterium.
- 社団法人日本生物工学会の論文
- 1987-04-25
著者
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NISHIZAWA YOSHINORI
Department of Fermentation Tachnology, Faculty of Engineering, Hiroshima University
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NAGAI SHIRO
Department of Fermentation Technology, Faculty of Engineering, Hiroshima University
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Nishizawa Yoshinori
Department Of Applied Chemistry And Chemical Engineering Faculty Of Engineering Yamaguchi University
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Nishizawa Yoshinori
Department Of Fermentation Technology Faculty Of Engineering Hiroshima University
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JEE HAE
Department of Fermentation Technology, Faculty of Engineering, Hiroshima University
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Nagai Shiro
Department Of Fermentation Technology Faculty Of Engineering Hiroshima University
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Jee Hae
Department Of Fermentation Technology Faculty Of Engineering Hiroshima University
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OHASHI TETSUYA
(Present address)Hayashibara Biochemical Laboratories, Inc.
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Ohashi Tetsuya
(present Address)hayashibara Biochemical Laboratories Inc.
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