Analysis of a Bacterial Community in 3-Chlorobenzoate-Contaminated Soil by PCR-DGGE Targeting the 16S rRNA Gene and Benzoate 1,2-Dioxygenase Gene (benA)
スポンサーリンク
概要
- 論文の詳細を見る
We analyzed the changes that occurred in a bacterial community from forest soil after adding 3-chlorobenzoate (3CB) by two different PCR-denaturing gradient gel electrophoreses (DGGEs), targeting the 16S rRNA gene and benA encoding the benzoate 1,2-dioxygenase large subunit. The concentration of 3CB in the soil had decreased to about 40% of that added (500ppm) after seven days at 28℃. Total DNA was directly extracted from the soil before and after incubation, and subjected to PCR-DGGE. Four new bands clearly appeared after the incubation with 3CB in the DGGE profiles of both the 16S rRNA gene and benA. The major bands were sequenced and compared with sequences obtained from DNA databases by phylogenetic analysis. All four of the new bands in the profiles of the 16S rRNA gene were most closely related to Burkholderia spp. Moreover, three of the four bands that intensified after the addition of 3CB in the profiles of benA were also most closely related to known benA sequences derived from Burkholderia strains. These results suggest that several Burkholderia-related bacteria played significant roles in the degradation of 3CB in the soil.
- 日本微生物生態学会の論文
著者
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藤井 毅
(独)農業環境技術研究所
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HASEBE Akira
National Institute of Agrobiological Resources (NIAR)
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Morimoto Sho
National Institute for Agro-Environmental Sciences
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Ogawa Naoto
National Institute for Agro-Environmental Sciences
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Fujii Takeshi
National Institute for Agro-Environmental Sciences
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Hasebe Akira
National Institute For Agro-environmental Sciences
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TOGAMI KAZUKI
National Institute for Agro-Environmental Sciences
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Fujii Takeshi
National Inst. For Agro-environmental Sciences
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Fujii Takeshi
Natl. Inst. Agro-environ. Sci.
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Morimoto Sho
National Inst. For Agro-environmental Sciences
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FUJII TAKESHI
Environmental Biofunction Division, National Institute for Agro-Environmental Sciences
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