Comparison among amoA Primers Suited for Quantification and Diversity Analyses of Ammonia-Oxidizing Bacteria in Soil
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概要
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Ammonia monooxygenase subunit A gene (amoA) is frequently used as a functional gene marker for diversity analysis of ammonia-oxidizing bacteria (AOB). To select a suitable amoA primer for real-time PCR and PCR-denaturing gradient gel electrophoresis (DGGE), three reverse primers (degenerate primer amoA-2R; non-degenerate primers amoA-2R-GG and amoA-2IR) were examined. No significant differences were observed among the three primers in terms of quantitative values of amoA from environmental samples using real-time PCR. We found that PCR-DGGE analysis with the amoA-2IR primer gave the best results in this studied soil. These results indicate that amoA-2IR is a suitable primer for community analysis of AOB in the environment.
- 2012-03-01
著者
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Morimoto Sho
National Institute for Agro-Environmental Sciences
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Yokoyama T
Tokyo University Of Agriculture And Technology
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AKIYAMA Hiroko
National Institute for Agro-Environmental Sciences
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Yokoyama Tadashi
Faculty Of Agriculture Tokyo University Of Agriculture And Technology
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Hayatsu Masahito
National Institute For Agro-environmental Sciences
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Takada Hoshino
National Inst. For Agro-environmental Sciences
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Morimoto Sho
National Inst. For Agro-environmental Sciences
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Shimomura Yumi
National Institute for Agro-Environmental Sciences
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Uchida Yoshitaka
National Institute for Agro-Environmental Sciences
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