The diacylated lipopeptide FSL-1 enhances phagocytosis of bacteria by macrophages through Toll-like receptor 2-mediated signaling pathway

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概要

• 論文の詳細を見る

• Enormous lines of evidence have been accumulated that Toll-like receptors (TLRs) function as sensors for microbial invasion. However, less is known about how signaling triggered by TLRs leads to phagocytosis of pathogens. This study was designed to determine whether stimulation of TLR2 with mainly the lipopeptide FSL-1 plays a role in phagocytosis of pathogens by macrophages. FSL-1 markedly enhanced phagocytosis of E. coli more strongly than that of S. aureus, but did not enhance phagocytosis of latex beads. FSL-1 stimulation resulted in enhanced phagocytosis of bacteria by macrophages from TLR2+/+mice but not those from TLR2-/- mice. Chinese hamster ovary cells stably expressing TLR2 failed tophagocytose these bacteria, but the cells expressing CD14 did. FSL-1 induced upregulation of the expression of phagocytic receptors including MSR1, CD36, DC-SIGN and Dectin-1 in THP-1 cells.Human embryonic kidney 293 cells transfected with DC-SIGN and MSR1 phagocytosed these bacteria. These results suggest that the FSL-1-induced enhancement of phagocytosis of bacteria by acrophages may be explained partially by the upregulation of scavenger receptors and the C-type lectins through TLR2-mediated signaling pathways and that TLR2 by itself does not function as a phagocytic receptor.

• Blackwellの論文
• 2007-02-22

著者

• Shibata K

  Department Of Oral Pathobiological Science Hokkaido University Graduate School Of Dental Medicine

• Hasebe Akira

  National Institute For Agro-environmental Sciences

• Hasebe A

  Aichi Univ. Education Kariya Jpn

• Hasebe Akira

  Department Of Environmental Chemistry National Institute For Agro-environmental Sciences

• Shibata Ken-ichiro

  Division Of Oral Molecular Microbiology Department Of Oral Pathobiological Science Hokkaido Universi

• Kiura Kazuto

  Division Of Oral Molecular Microbiology Department Of Oral Pathobiological Science Hokkaido Universi

• Hasebe Akira

  Division Of Oral Molecular Microbiology Department Of Oral Pathobiological Science Hokkaido Universi

• Totsuka Yasunori

  Division Of Oral And Maxillofacial Surgery Department Of Oral Pathobiological Science Hokkaido Unive

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