The diacylated lipopeptide FSL-1 induces TLR2-mediated Th2 responses
スポンサーリンク
概要
- 論文の詳細を見る
The diacylated lipopeptide FSL-1 enhanced the generation of IgG antibodies in TLR2+/+ mice, but not in TLR2-/- mice, when administered together with hen egg lysozyme as an antigen. Escherichia coli lipopolysaccharide enhanced the generation of antigen-specific antibodies in both TLR2-/- and TLR2+/+ mice. In TLR2+/+ mice, the enhancement level by FSL-1 was similar to that by lipopolysaccharide. Analysis of the IgG antibodies subclass demonstrated that level of Th2-type IgG1 antibodies was higher than that of Th1-type IgG2a antibodies. Both FSL-1 and lipopolysaccharide induced production of IL-10 and IL-6 by splenocytes from TLR2+/+ mice. Lipopolysaccharide also induced production of these cytokines by splenocytes from TLR2-/- mice, but FSL-1 did not. Neither FSL-1 nor lipopolysaccharide induced IL-12p70 production by splenocytes from both types of mice. FSL-1 upregulated B7.2 expression in B220+ cells from TLR2+/+ mice but not those from TLR2-/- mice, whereas lipopolysaccharide upregulated B7.2 expression in B220+ cells from both types of mice. FSL-1 and, to a lesser extent, lipopolysaccharide activated mitogen-activated protein kinases in splenocytes. FSL-1 and, to a lesser extent,lipopolysaccharide induced the expression of c-Fos, which is known to be involved in Th2-type responses, in splenocytes. Thus, this study demonstrated that FSL-1 possessed TLR2-mediated Th2-type responses in vivo.44
- 55の論文
著者
-
Shibata K
Department Of Oral Pathobiological Science Hokkaido University Graduate School Of Dental Medicine
-
Shibata Ken-ichiro
Division Of Oral Molecular Microbiology Department Of Oral Pathobiological Science Hokkaido Universi
-
Kiura Kazuto
Division Of Oral Molecular Microbiology Department Of Oral Pathobiological Science Hokkaido Universi
-
Shibata Ken-ichiro
Division Of Oral Molecular Microbiology Department Of Oral Pathobiological Science Hokkaido Universi
関連論文
- The TLR2 ligand FSL-1 is internalized via clathrin-dependent endocytic pathway triggered by CD14 and CD36 but not by TLR2
- A role of the Ca2+ binding site of DC-SIGN in the phagocytosis of E. coli
- The diacylated lipopeptide FSL-1 enhances phagocytosis of bacteria by macrophages through Toll-like receptor 2-mediated signaling pathway
- Resveratrol modulates phagocytosis of bacteria through an NF-κB-dependent gene program
- CD14 directly binds to triacylated lipopeptides and facilitates recognition of the lipopeptides by the receptor complex of Toll-like receptors 2 and 1 without binding to the complex
- The diacylated lipopeptide FSL-1 induces TLR2-mediated Th2 responses
- Roles of N-linked glycans in the recognition of microbial lipopeptides and lipoproteins by TLR2
- The synthetic analogue of mycoplasmal lipoprotein FSL-1 induces dendritic cell maturation through
- Extracellular ATP Regulates Cell Death of Lymphocytes and Monocytes Induced by Membrane-Bound Lipoproteins of Mycoplasma fermentans and Mycoplasma salivarium
- Mycoplasmal Lipoproteins Induce Toll-Like Receptor 2- and Caspases-Mediated Cell Death in Lymphocytes and Monocytes
- Signaling Pathways Induced by Lipoproteins Derived from Mycoplasma salivarium and a Synthetic Lipopeptide (FSL-1) in Normal Human Gingival Fibroblasts
- Partial Purification and Characterization of the Active Entity Responsible for Inducing Interleukin-6 Production by Human Gingival Fibroblasts from Mycoplasma salivarium Cells
- Detection of Mycoplasma fermentans in Saliva Sampled from Infants, Preschool and School Children, Adolescents and Adults by a Polymerase Chain Reaction-Based Assay
- Relationship between structures and biological activities of mycoplasmal diacylated lipopeptides and their recognition by Toll-like receptors 2 and 6
- Synergic effects of mycoplasmal lipopeptides and extracellular ATP on activation of macrophages
- Structure-function Relationship of Mycoplasmal Lipoproteins/lipopeptides and Their Recognition by Toll-like Receptor 2
- Relationship between expression and function of Toll-like receptor 2 and 4 with aging in humans and in mice
- Roles of the Src family kinase Lck in Toll-like receptor 2 signaling triggered by stimulation with a mycoplasma derived lipopeptide
- Involvement of SOCS-1-mediated degradation of mal in the suppression of TLR2-mediated signaling by DC-SIGN-mediated signaling
- In vivo anti- and pro-tumor activities of the mycoplasmal lipopeptide FSL-1 and Mycoplasma pneumoniae cells