Synergic effects of mycoplasmal lipopeptides and extracellular ATP on activation of macrophages
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概要
- 論文の詳細を見る
Mycoplasmal lipopeptides, S-(2,3-bispalmitoyloxypropyl)-CGDPKHSPKSF and S-(2,3-bispalmitoyloxypropyl)-CGNNDESNISFKEK activated a monocytic cell line,THP-1 cells, to produce tumor necrosis factor-α. The activity of the lipopeptides was augmented by ATP added in a dose-dependent manner. In addition, the level of the expression of mRNAs of tumor necrosis factor-α, interleukin-1β, -6 and -8 was also up-regulated by the lipopeptides and/or extracellular ATP, but that of interleukin-10 was not. The P2X purinergic receptor antagonists pyridoxal phosphate 6-azophenyl 2’,4’-disulfonic acid and periodate oxidized ATP suppressed the activity of ATP to augment the activation of THP-1 cells by the lipopeptides, suggesting that P2X receptors play important roles in the activity of ATP. The nuclear factor-κB inhibitor dexamethasone also suppressed the activity,suggesting that the activity of ATP is dependent upon the nuclear factor-κB.Thus, these results suggest that the interaction of extracellular ATP with the P2X receptors is attributed to the activity of ATP to augment the activation of THP-1 cells by mycoplasmal lipopeptides.
- American Society For Microbiologyの論文
著者
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Shibata K
Department Of Oral Pathobiological Science Hokkaido University Graduate School Of Dental Medicine
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Fujita Mari
Department Of Oral Health Science Hokkaido University Graduate School Of Dental Medicine
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Hasebe Akira
National Institute For Agro-environmental Sciences
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Hasebe A
Aichi Univ. Education Kariya Jpn
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Hasebe Akira
Department Of Environmental Chemistry National Institute For Agro-environmental Sciences
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Morita Manabu
Department Of Oral Health Science Hokkaido University Graduate School Of Dental Medicine
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Shibata Ken-ichiro
Division Of Oral Molecular Microbiology Department Of Oral Pathobiological Science Hokkaido Universi
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Hasebe Akira
Division Of Oral Molecular Microbiology Department Of Oral Pathobiological Science Hokkaido Universi
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Shibata Ken-ichiro
Division Of Oral Molecular Microbiology Department Of Oral Pathobiological Science Hokkaido Universi
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