Resveratrol modulates phagocytosis of bacteria through an NF-κB-dependent gene program
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概要
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Many studies have shown that the pharmacological effects of resveratrol, a phytoalexin polyphenolic compound, include protective effects against cancer and inflammation as well as enhancement of stress resistance. In this study, we examined whether resveratrol affected phagocytosis of bacteria by macrophages and activation of the transcription factor NF-κB after stimulation with or without the ligand FSL-1 for Toll-like receptor 2 (TLR2). Both phagocytosis of E. coli or S. aureus by THP-1 cells or RAW264.7 cells was inhibited by resveratrol in a dose-dependent manner regardless of stimulation with FSL-1. The NF-κB activity in HEK293 cells stably expressing TLR2 was also inhibited by resveratrol after stimulation with FSL-1. Resveratrol also inhibited both the translocation of p65 of NF-κB into nuclei in the transfectant and TNF-α production by THP-1 cells or RAW264.7 cells. It has recently been reported that TLR-mediated signaling pathways lead to upregulation of mRNAs of phagocytic receptors, including scavenger receptors and C-type lectin receptors.This study also demonstrated that FSL-1 induced upregulation of mRNAs of phagocytic receptors such as macrophage scavenger receptor-1, CD36, DC-SIGN and Dectin-1, and the FSL-1-induced upregulation of their mRNAs was inhibited by resveratrol. In addition, it was found that the expression of DC-SIGN in HEK293 cells stably expressing DC-SIGN was reduced by resveratrol and the phagocytic activity was significantly inhibited by resveratrol. Thus, this study suggested that resveratrol inhibited the bacterial phagocytosis by macrophages by downregulating the expression of phagocytic receptors and the NF-κB activity.
- American Society for Microbiologyの論文
- 2007-02-22
著者
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Shibata K
Department Of Oral Pathobiological Science Hokkaido University Graduate School Of Dental Medicine
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Hasebe Akira
National Institute For Agro-environmental Sciences
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Hasebe A
Aichi Univ. Education Kariya Jpn
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Hasebe Akira
Department Of Environmental Chemistry National Institute For Agro-environmental Sciences
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Shibata Ken-ichiro
Division Of Oral Molecular Microbiology Department Of Oral Pathobiological Science Hokkaido Universi
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Kiura Kazuto
Division Of Oral Molecular Microbiology Department Of Oral Pathobiological Science Hokkaido Universi
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Hasebe Akira
Division Of Oral Molecular Microbiology Department Of Oral Pathobiological Science Hokkaido Universi
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