Finding of UDP-Glucose : Nicotinic Acid-N-glucosyltransferase Activity in Cultured Tobacco Cells and Its Properties
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概要
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We have already identified N-(β-D-glucopyranosyl)nicotinic acid, which was isolated from cultured tobacco cells, as a novel niacin metabolite. The enzyme activity for the biosynthesis of this compound, UDP-glucose: nicotinic acid-N-glucosyltransferase, was found in cell-free extracts from cultured cells of tobacco (Nicotiana tabacum XD-6) and properties were investigated in this experiment. The general catalytic properties were as follows: optimum temperature was 25℃, optimum pH was 6.1, the K_m for NiA and UDP-glucose were calculated at 220 μM and 4.3 mM, respectively. The enzyme activity was lowered by heavy metal ions, isonicotinic acid, benzoic acid, and 6-hydroxynicotinic acid. The roles of niacin metabolites in cultured tobacco cells are discussed in relation to the enzyme.
- 社団法人日本農芸化学会の論文
- 1997-04-23
著者
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Okumura Katsuzumi
Laboratory Of Biological Chemistry Faculty Of Bioresources Mie University
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NISHITANI Hiroshi
R & D Center, Terumo Corporation
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Taguchi Hiroshi
Laboratory Of Biological Chemistry Faculty Of Bioresources Mie University
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Taguchi H
Department Of Bioresource Development Hiroshima Prefectural University
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Nishitani Hiroshi
R & D Center Terumo Corporation
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SASATANI Kazuyoshi
Laboratory of Biological Chemistry, Faculty of Bioresources, Mie University
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Sasatani Kazuyoshi
Laboratory Of Biological Chemistry Faculty Of Bioresources Mie University
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