A Null Mutant of the Streptomyces virginiae barA Gene Encoding a Butyrolactone Autoregulator Receptor and Its Phenotypic and Transcriptional Analysis

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概要

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• The Streptomyces virginiae barA gene encodes a specific receptor protein for virginiae butanolide(VB), one of the γ-butyrolactone autoregulators of Streptomyces species. By homologous recombination, a barA null strain was constructed to clarify the in vivo function of BarA protein in S.virginiae. The ΔbarA mutant showed no difference in terms of growth, but lost VB production and produced virginiamycin 7 h earlier than the wild-type strain. These results indicated that, phenotypically, BarA protein acts negatively in virginiamycin biosynthesis and positively in VB biosynthesis. Furthermore, Northern (RNA) blot analysis of the ΔbarA mutant revealed that transcription of the BarA target gene (barB) was derepressed, confirming that BarA acts as a transcriptional repressor in S.virginiae.

• 社団法人日本生物工学会の論文
• 2000-08-25

著者

• Yamada Yasuhiro

  Department Of Biotechnology Osaka University

• Lee Chang-kwon

  Department Of Physiology & Immunology School Of Medicine Konkuk University

• Nihira Takuya

  Department of Fermentation Technology, Faculty of Engineering, Osaka University

• Nihira Takuya

  Department Of Biotechnology Faculty Of Engineering Osaka University

• Lee Chang-kwon

  Department Of Biotechnology Graduate School Of Engineering Osaka University

• NAKANO HIROKO

  Department of Biotechnology, Graduate School of Engineering, Osaka University

• Nakano Hiroko

  Department Of Biotechnology Graduate School Of Engineering Osaka University

• Yamada Yasuhiro

  Department Of Agricultural Chemistry Faculty Of Agriculture University Of Tokyo

• Nihira Takuya

  Department of Biotechnology, Graduate School of Engineering, Osaka University

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