Increased Production of Lactonizing Lipase (LipL) from Pseudomonas sp. strain 109 by Lipids and Detergents
スポンサーリンク
概要
- 論文の詳細を見る
LipL of Pseudomonas sp. strain 109 is a unique lipase capable of catalyzing macrocyclic lactone synthesis using ω-hydroxyfatty acid esters as substrates. Several fatty acid esters were tested as inducers of LipL production. The addition of either soybean oil or a non-ionic detergent (Noigen HC) resulted in a 44 to 45-fold increase in extracellular LipL, and the presence of both resulted in a further 56-fold increase, Among the triglycerides tested, triolein was the most effective, with a 50-fold increase in LipL production. A Northern blot hybridization analysis found that the lipL transcript increased in the presence of soybean oil or Noigen HC, indicating that the production of LipL is regulated at the transcriptional level.
- 社団法人日本農芸化学会の論文
- 1999-05-23
著者
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Yamada Yasuhiro
Department Of Biotechnology Osaka University
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TANAKA Junko
Department of Medicine, Kidney Center, Tokyo Women's Medical University
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Yamada Y
Osaka Univ. Osaka Jpn
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Tanaka Junko
Department Of Biotechnology Graduate School Of Engineering Osaka University
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Nihira Takuya
Department of Fermentation Technology, Faculty of Engineering, Osaka University
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Ihara Fumio
Department Of Plant Protection National Institute Of Fruit Tree Science
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Ihara Fumio
Apple Pest Control Research Subteam National Institute Of Fruit Tree Science
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Nihira T
Osaka Univ. Osaka Jpn
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Nihira Takuya
Department Of Biotechnology Faculty Of Engineering Osaka University
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Yamada Y
Department Of Biotechnology Graduate School Of Engineering Osaka University
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SUDO Towa
Department of Biotechnology, Graduate School of Engineering, Osaka University
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Sudo Towa
Department Of Biotechnology Graduate School Of Engineering Osaka University
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Nihira T
International Center For Biotechnology Osaka University
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Yamada Yasuhiro
Laboratory Of Molecular Biotechnology Division Of Molecular And Cellular Mechanisms Graduate School
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Yamada Yasuhiro
Department Of Agricultural Chemistry Faculty Of Agriculture University Of Tokyo
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