Enhanced Productivity of Human Lysozyme by pH-Controlled Batch Fermentation of Recombinant Saccharomyces cerevisiae(BREWING AND FOOD TECHNOLOGY)

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概要

• 論文の詳細を見る

• Human lysozyme (HLY) was successfully produced by Saccharomyces cerevisiae with a plasmid (pHK501) containing a synthetic HLY gene connected with a native secretion-signal sequence under control of the promoter for the glyceraldehydes-3-phosphate dehydrogenase gene. For large-scale production, batch fermentation was adopted with 1lSD (Leu^- ) minimal medium in a 2-l jar fermentor. While pH-uncontrolled fermentation gave 42.3 units/ml of HLY over a period of 72-h cultivation, HLY production increased to 74.5 units/ml by performing pH-controlled fermentation, although a longer fermentation time was needed for maximal HLY production. A pH-shift strategy was designed in this study, resulting in higher HLY production and a shorter fermentation time.

• 社団法人日本生物工学会の論文
• 2004-08-25

著者

• Nihira Takuya

  International Center for Biotechnology, Osaka University

• Choi S‐u

  International Center For Biotechnology Osaka University

• Nihira T

  Osaka Univ. Osaka Jpn

• Nihira Takuya

  Department Of Biotechnology Osaka University

• Nihira Takuya

  International Center For Biotechnology Osaka University

• Lee Seung-cheol

  Division Of Food And Biotechnology Kyungnam University

• Nakamura Takefumi

  Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni

• Nihira T

  International Center For Biotechnology Osaka University

• CHOI SUN-UK

  International Center for Biotechnology, Osaka University

• PAIK HYN-DONG

  Division of Food and Biotechnology, Kyungnam University

• WANG YONG-IL

  Division of Food and Biotechnology, Kyungnam University

• Choi Sun-uk

  International Center For Biotechnology Osaka University:(present Address)division Of Food And Biotec

• Wang Yong-il

  Division Of Food And Biotechnology Kyungnam University

• Paik Hyn-dong

  Division Of Food And Biotechnology Kyungnam University:(present Address)department Of Food Science A

• PAIK Hyun-Dong

  Division of Animal Life Science, Konkuk University

• Nihira Takuya

  International Center For Biotechnology Osaka Univ.

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