Requirement in trans of the Downstream limL Gene for Activation of Lactonizing Lipase form Pseudomonas sp. 109
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概要
- 論文の詳細を見る
A novel lipase (lactonizing lipase : lipL) from Pseudomonas sp. 109 can catalyze synthesis of macrovyclic lactones in anhydrous organic solvents by transesterification. A gene situated immediately downstream from lipL was found to be essential for production of the functional lipase, and was denoted as limL (lipase modulator). Nucleotide sequence analysis of the limL region revealed a 340-amino acid open reading frame (M_r=37,658) 49 bp downstream from lipL. In the non-coding region between lipL and limL was situated a 32-base strong inverted repeat, and a putative ribosome binding site was present at 14 bases upstream from the limL initiator methionine. The deduced amino-acid sequence of LimL exhibited 29% overall homology with that of the Iipase-modulator protein (LimA) of Pseudomonas cepacia. Although the homology itself was low, hydrophobicity/hydrophilicity plots of the two modulator proteins showed that the two proteins shared a similar hydropathy pattern, i.e., the amino-terminus region is highly hydrophobic and the carboxyl-terminus region is strongly hydrophilic, suggesting that the two modulator proteins may activate corresponding lipase by similar functions. When lipL and limL were cloned separately in compatible plasmids, functional lipase was produced only in E. coli hosts harboring both the plasmids, indicating that limL can act in trans toward lipL.
- 社団法人日本生物工学会の論文
- 1992-05-25
著者
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Yamada Yasuhiro
Department Of Biotechnology Osaka University
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Yamada Y
Osaka Univ. Osaka Jpn
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Nihira Takuya
Department of Fermentation Technology, Faculty of Engineering, Osaka University
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Ihara Fumio
Department Of Plant Protection National Institute Of Fruit Tree Science
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Ihara Fumio
Apple Pest Control Research Subteam National Institute Of Fruit Tree Science
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Nihira T
Osaka Univ. Osaka Jpn
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Nihira Takuya
Department Of Biotechnology Faculty Of Engineering Osaka University
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Yamada Y
Department Of Biotechnology Graduate School Of Engineering Osaka University
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OKAMOTO IWAO
Department of Biotechnology, Faculty of Engineering, Osaka University
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Nihira T
International Center For Biotechnology Osaka University
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Okamoto Iwao
Department Of Biotechnology Faculty Of Engineering Osaka University
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Yamada Yasuhiro
Laboratory Of Molecular Biotechnology Division Of Molecular And Cellular Mechanisms Graduate School
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Yamada Yasuhiro
Department Of Agricultural Chemistry Faculty Of Agriculture University Of Tokyo
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