Immunological Properties of Sedoheptulose-1,7-bisphosphatase from Chlamydomonas sp. W80
スポンサーリンク
概要
- 論文の詳細を見る
Here we report on the production of functional recombinant SBPase of Chlamydomonas sp. W80 in Escherichia coli and the one-step purification of a polyhistidine-tagged fusion protein. The polyclonal antibody was raised against purified recombinant enzyme and cross-reacted with crude SBPase from Chlamydomonas, spinach, tobacco, and Arabidopsis leaves. Further, we investigated the levels of protein and activity of SBPase in different tissues of Arabidopsis plants.
- 社団法人 日本農芸化学会の論文
- 2005-04-23
著者
-
Sano Satoshi
Research Institute Of Innovative Technology For The Earth (rite):(present Address)faculty Of Biologi
-
Maruta Takanori
Dep. Of Advanced Bioscience Fac. Of Agriculture Kinki Univ.
-
Tamoi Masahiro
Department Of Advanced Bioscience Faculty Of Agriculture Kinki University
-
Shigeoka Shigeru
Department Of Advanced Bioscience Faculty Of Agriculture Kinki University
-
Shigeoka Shigeru
Dept. Adv. Biosci. Fac. Agr. Kinki Univ
-
Maruta Takanori
Department Of Food And Nutrition Faculty Of Agriculture Kinki University
-
NAGAOKA Miki
Department of Advanced Bioscience, Faculty of Agriculture, Kinki University
-
Nagaoka Miki
Department Of Advanced Bioscience Faculty Of Agriculture Kinki University
関連論文
- Floricultural Salvia plants have a high ability to eliminate bisphenol A(ENVIRONMENTAL BIOTECHNOLOGY)
- Effect of the sequence context of the AUG initiation codon on the rate of translation in dicotyledonous and monocotyledonous plant cells(PLANT BIOTECHNOLOGY)
- Comparative analysis of a CFo ATP synthase subunit II homologue derived from marine and fresh-water algae(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
- Isolation and characterization of a novel antistress gene from Chlamydomonas sp. W80(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
- RESISTANCE TO ENVIRONMENTAL STRESS IN TRANSGENIC TOBACCO OVEREXPRESSING OF CATALASE FROM E. coli IN CHLOROPLASTS
- Occurrence of Pseudovitamin B_ and Its Possible Function as the Cofactor of Cobalamin-Dependent Methionine Synthase in a Cyanobacterium Synechocystis sp. PCC6803
- COMPARISON OF REGULATION MECHANISM OFTHIOL ENZYMES INVOLVED IN THE PHOTOSYNTHETIC CAARBON REDUCTIONCYCLE BETWEEN ALGAE AND HIGHER PLANT
- Feedback Inhibition of Spinach L-Galactose Dehydrogenase by L-Ascorbate
- Crystal Structure of Chloroplastic Ascorbate Peroxidase from Tobacco Plants and Structural Insights into its Instability
- Characterization of Ascorbate Peroxidases from Unicellular Red Alga Galdieria partita
- REGULATORY MECHANISMS OF THE CALVIN CYCLE IN CYANOBACTERIA
- THE ROLE OF SELENIUM FOR THE INDUCTION OF GLUTATHIONE PEROXIDASE IN Chlamydomonas reinhardtii
- Molecular Characterization of Tobacco Mitochondrial L-Galactono-γ-Lactone Dehydrogenase and Its Expression in Escherichia coli
- ANALYSIS OF PHYSIOLOGICAL FUNCTIONS OF CATALASE-PEROXIDASE IN Synechococcus PCC 7942 USING GENE-INTERRUPTING MUTANT
- Functional Analysis of Fructose-1, 6-Bisphosphatase Isozymes (fbp-I and fbp-II Gene Products) in Cyanobacteria
- GENE REGULATION OF CHLOROPLASTIC ASCORBATE PEROXIDASE ISOZYMES BY ALTERNATIVE mRNA SPLICING OF 3'-TERMINAL EXONS IN SPINACH
- Lack of Ligth/Dark Regulation of Enzymes Involved in the Photosynthetic Carbon Reduction Cycle in Cyanobacteria, Synechococcus PCC 7942 and Synechocystis PCC 6803
- Molecular Characterization and Physiological Role of a Glyoxysome-Bound Ascorbate Peroxidase from Spinach
- THE SPINACH CHLOROPLASTIC ASCORBATE PEROXIDASE GENE PRODUCES mRNAs FOR ISOZYMES BY ALTERNATIVE SPLICING AT 3'-TERMINAL EXONS
- MOLECULAR CHARACTERIZATION AND PHYSIOLOGICAL ROLE OF A MICROBODY-BOUND ASCORBATE PEROXIDASE FROM SPINACH
- THE MOLECULAR CHARACTERIZATION OF A NEW TYPE OF FRUCTOSE-1, 6-BISPHOSPHATASE IN Synechocystis PCC6803
- High-Efficiency Secretory Production of Peroxidase Cla Using Vesicular Transport Engineering in Transgenic Tobacco(PLANT BIOTECHNOLOGY)
- 2-III-22 Intermolecular dimmer structure of Euglena ascorbate peroxidase
- Functional Characterization of D-Galacturonic Acid Reductase, a Key Enzyme of the Ascorbate Biosynthesis Pathway, from Euglena gracilis
- Two Distinct Redox Signaling Pathways for Cytosolic APX Induction under Photooxidative Stress
- Response of the antioxidative system in chloroplasts of transgenic tobacco plants overexpressing thylakoid-membrane-bound ascorbate peroxidase under photooxidative stress
- Stable Form of Ascorbate Peroxidase from the Red Alga Galdieria partita Similar to Both Chloroplastic and Cytosolic Isoforms of Higher Plants(Biochemistry & Molecular Biology)
- PROPERTIES OF ASCORBATE PEROXIDASE FROM A UNICELLULAR RED ALGA, Galdieria partita
- Differential Expression of Alternatively Spliced mRNAs of Arabidopsis SR Protein Homologs, atSR30 and atSR45a, in Response to Environmental Stress
- AtNUDX1, and 8-Oxo-7,8-Dihydro-2'-Deoxyguanosine 5'-Triphosphate Pyrophosphohydrolase, is Responsible for Eliminating Oxidized Nucleotides in Arabidopsis
- Phytochelatins inhibit the metal-induced aggregation of α-crystallin(PLANT BIOTECHNOLOGY)
- 26kDa Endochitinase from Barley Seeds : An Interaction of the Ionizable Side Chains Essential for Catalysis
- CLONING AND SEQUENCE ANALYSIS OF A cDNA ENCODING CHLOROPLASTIC ASCORBATE PEROXIDASE FROM SPINACH
- CLONING AND CHARACTERIZATION OF 1.2kbp-cDNA ENCODING SPINACH PEROXIDASE
- ENZYMATIC AND MOLECULAR CHARACTERIZATIONOF CATALASE IN Synechococcus PCC 7942
- CHARACTERIZATION OF ASCORBATE PEROXIDASEFROM EUGLENA GRACILIS Z
- THE MOLECULAR CHARACTERIZATION OFH_2 O_2-RESISTANCE SYSTEM OF FRUCTOSE-1,6-BISPHOSPHATASE IN Synechococcus PCC7942
- ENZYMOLOGICAL AND MOLECULARCHARACTERIZATION OF NADP-GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASEFROM Synechococcus PCC7942
- The Presence of Enzymes Related to Glutathione Metabolism and Oxygen Metabolism in Chlamydomonas reinhardtii
- Chitinase Gene Expression in Response to Environmental Stresses in Arabidopsis thaliana : Chitinase Inhibitor Allosamidin Enhances Stress Tolerance
- Molecular Characterization and Redox Regulation of Phosphoribulokinase from the Cyanobacterium Synechococcus sp. PCC 7942
- The 26S Proteasome Function and Hsp90 Activity Involved in the Regulation of HsfA2 Expression in Response to Oxidative Stress
- Analysis of the Regulation of Target Genes by an Arabidopsis Heat Shock Transcription Factor, HsfA2
- The Contribution of Arabidopsis Homologs of L-Gulono-1,4-lactone Oxidase to the Biosynthesis of Ascorbic Acid
- Arabidopsis Chloroplastic Ascorbate Peroxidase Isoenzymes Play a Dual Role in Photoprotection and Gene Regulation under Photooxidative Stress
- Conversion of L-Galactono-1,4-lactone to L-Ascorbate Is Regulated by the Photosynthetic Electron Transport Chain in Arabidopsis
- 1-III-3 Vitamin C Biosynthesis and Translocation in Tomato (Lycopersicum esculentum) Fruits
- Transgenic Lettuce Producing a Candidate Protein for Vaccine against Edema Disease
- L-GALACTONO-γ-LACTONE DEHYDROGENASE IS COUPLED WITH THE RESPIRATORY ELECTRON TRANSPORT SYSTEM
- GENE EXPRESSION OF CHLOROPLASTIC ASCORBATE PEROXIDASE ISOENZYMES IN TOBACCO PLANTS
- Arabidopsis NAC Transcription Factor, ANAC078, Regulates Flavonoid Biosynthesis under High-light
- Molecular Cloning and Partial Characterization of a Peroxidase Gene Expressed in the Roots of Portulaca oleracea cv., One Potentially Useful in the Remediation of Phenolic Pollutants
- Molecular Design of Photosynthesis-Elevated Chloroplasts for Mass Accumulation of a Foreign Protein
- Carbon metabolism in the Calvin cycle
- MOLECULAR CHARACTERIZATION OF FRUCTOSE-1, 6-BISPHOSPHATASE ISOZYMES FROM Synechococcus PCC7942
- Enzymic and molecular characterization of NADP-dependent glyceraldehyde-3-phosphate dehydrogenase from Synechococcus PCC7942:resistance of the enzyme to hydrogen peroxide
- Subcellular Localization and Properties of L-Galactono-γ-lactone Dehydrogenase in Spinach Leaves
- Contribution of Fructose-1,6-bisphosphatase and Sedoheptulose-1,7-bisphosphatase to the Photosynthetic Rate and Carbon Flow in the Calvin Cycle in Transgenic Plants
- Immunological Properties of Sedoheptulose-1,7-bisphosphatase from Chlamydomonas sp. W80
- ANALYSIS OF CARBON METABOLISM IN SOURCE AND SINK ORGANS OF TRANSGENIC TOBACCO PLANT HAVING CYANOBACTERIAL FBP/SBPase IN CHLOROPLASTS OR CYTOSOL
- Evaluation of the Defense System in Chloroplasts to Photooxidative Stress Caused by Paraquat Using Transgenic Tobacco Plants Expressing Catalase from Escherichia coli
- ANALYSIS OF PHOTOSYNTHETIC CAPACITY AND CARBON METABOLISM IN TRANSGENIC TOBACCO PLANT HAVING CYANOBACTERIAL FBP/SBPase IN CHLOROPLASTS
- REGULATORY MECHANISMS OF PHOTOSYNTHETIC CARBON REDUCTION CYCLE IN CYANOBACTERIA : Physiological Functions of CP12
- MODULATION OF CARBON METABOLISM IN HIGHER PLANTS WITH IMPROVED CAPACITY IN PHOTOSYNTHESIS BY INTRODUCTION OF CYANOBACTERIAL FBP/SBPase INTO CHLOROPLASTS
- ANALYSIS OF PHOTOSYNTHESIS AND CARBON METABOLISM IN TRANSGENIC TOBACCO PLANT HAVING CYANOBACTERIAL FBP/SBPase IN CHLOROPLASTS
- Recent Advances in Ascorbate Biosynthesis and the Physiological Significance of Ascorbate Peroxidase in Photosynthesizing Organisms
- THE REQUIREMENT OF SELENIUM FOR THE INDUCTION OF GLUTATHIONE PEROXIDASE-LIKE PROTEIN IN Chlamydomonas reinhardtii
- ANALYSIS OF RESISTANCE MECHANISM TO PHOTOOXIDATIVE STRESS IN TRANSGENIC TOBACCO OVEREXPRESSING OF CATALASE FROM E. coli IN CHLOROPLASTS
- Expression Analysis of the VTC2 and VTC5 Genes Encoding GDP-L-Galactose Phosphorylase, an Enzyme Involved in Ascorbate Biosynthesis, in Arabidopsis thaliana
- Comparison of three Chlamydomonas strains which show distinctive oxidative stress tolerance(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
- Expression Analysis of the VTC2 and VTC5 Genes Encoding GDP-L-Galactose Phosphorylase, an Enzyme Involved in Ascorbate Biosynthesis, in Arabidopsis thaliana
- Activity of the C-terminal-Dependent Vacuolar Sorting Signal of Horseradish Peroxidase C1a is Enhanced by its Secondary Structure
- HafA1d and HsfA1e Involved in the Transcriptional Regulation of HsfA2 Function as Key Regulators for the Hsf Signaling Network in Response to Environmental Stress
- REGULATION OF HYDROPEROXIDES-SCAVENGING SYSTEM IN THE HALOPHYTE Mesembryanthemum Crystallinum
- Identification of Alternative Splicing Events Regulated by an Arabidopsis Serine/Arginine-Like Protein, atSR45a, in Response to High-Light Stress using a Tiling Array
- Resistance of Photosynthesis to Hydrogen Peroxide in Algae : ENVIRONMENTAL AND STRESS RESPONSES : PROTEINS, ENZYMES AND METABOLISM
- A Comparative Analysis of the Molecular Characteristics of the Arabidopsis CoA Pyrophosphohydrolases AtNUDX11, 15, and 15a
- An Arabidopsis FAD Pyrophosphohydrolase, AtNUDX23, is Involved in Flavin Homeostasis
- High level expression of transgenes by use of 5'-untranslated region of the Arabidopsis thaliana arabinogalactan-protein 21 gene in dicotyledons
- Isolation of polyphenol oxidase genes from Portulaca oleracea and evaluation of their ability to metabolize endocrine-disrupting chemicals
- Characterization of Bisphenol A Metabolites Produced by Portulaca oleracea cv. by Liquid Chromatography Coupled with Tandem Mass Spectrometry
- Effects of Air Flow on Production of Vaccine Protein against Swine Edema Disease in Transgenic Lettuce
- The Involvement of Arabidopsis Glutathione Peroxidase 8 in the Suppression of Oxidative Damage in the Nucleus and Cytosol
- Isolation of polyphenol oxidase genes from Portulaca oleracea and evaluation of their ability to metabolize endocrine-disrupting chemicals
- Subcellular and Subnuclear Distribution of High-Light Responsive Serine/Arginine-Rich Proteins, atSR45a and atSR30, in Arabidopsis thaliana
- Effects of Diets Initiated at the Hypertensive Stage on SHRSP Blood Pressures, Life-spans and Incidences of Stroke Lesions
- Occurrence and Subcellular Distribution of Thiamine Pyrophosphokinase Isozyme in Euglena gracilis
- The Contents and Subcellular Distribution of Tocopherols in Euglena gracilis
- Photoinduced Biosynthesis of Glutathione in Euglena gracilis
- Enzymatic and Molecular Characterization of Arabidopsis ppGpp Pyrophosphohydrolase, AtNUDX26
- Activation of γ-Aminobutyrate Production by Chloroplastic H_2O_2 Is Associated with the Oxidative Stress Response