Functional Characterization of D-Galacturonic Acid Reductase, a Key Enzyme of the Ascorbate Biosynthesis Pathway, from Euglena gracilis

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概要

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• D-Galacturonic acid reductase, a key enzyme in ascorbate biosynthesis, was purified to homogeneity from Euglena gracilis. The enzyme was a monomer with a molecular mass of 38–39 kDa, as judged by SDS–PAGE and gel filtration. Apparently it utilized NADPH with a Km value of 62.5±4.5 μM and uronic acids, such as D-galacturonic acid (Km=3.79±0.5 mM) and D-glucuronic acid (Km=4.67±0.6 mM). It failed to catalyze the reverse reaction with L-galactonic acid and NADP+. The optimal pH for the reduction of D-galacturonic acid was 7.2. The enzyme was activated 45.6% by 0.1 mM H2O2, suggesting that enzyme activity is regulated by cellular redox status. No feedback regulation of the enzyme activity by L-galactono-1,4-lactone or ascorbate was observed. N-terminal amino acid sequence analysis revealed that the enzyme is closely related to the malate dehydrogenase families.

• 社団法人 日本農芸化学会の論文
• 2006-11-23

著者

• Ishikawa Tomohiro

  Department Of Agricultural Chemistry School Of Agriculture Nagoya University

• Sano Satoshi

  Research Institute Of Innovative Technology For The Earth (rite):(present Address)faculty Of Biologi

• SHIBATA Hitoshi

  Department of Life Science and Biotechnology, Faculty of Life and Environmental Science, Shimane Uni

• NISHIKAWA Hitoshi

  Department of Chest Surgery, Niigata Rosai Hospital

• Shibata Hiroyuki

  Institute For Chemical Research Kyoto University

• ISHIKAWA Takahiro

  Department of Life Science and Biotechnology, Faculty of Life and Environmental Science, Shimane Uni

• SAWA Yoshihiro

  Department of Life Science and Biotechnology, Faculty of Life and Environmental Science, Shimane Uni

• SHIGEOKA Shigeru

  Department of Advanced Bioscience, Faculty of Agriculture, Kinki University

• Yabuta Yukinori

  School Of Agricultural Biological And Environmental Sciences Fac. Of Agriculture Tottori Univ.

• YABUTA Yukinori

  Department of Food and Nutrition, Faculty of Agriculture, Kinki University

• Ishikawa T

  Fac. Of Life And Environmental Sci. Shimane Univ.

• MASUMOTO Ikuko

  Department of Life and Environmental Sciences, Shimane University

• IWASA Naofumi

  Department of Life and Environmental Sciences, Shimane University

• NAKAMURA Ayana

  Department of Advanced Bioscience, Faculty of Agriculture, Kinki University

• Iwasa Naofumi

  Department Of Life And Environmental Sciences Shimane University

• Shibata H

  Dept. Life Environ. Sci. Shimane Univ.

• Sawa Yoshihiro

  Dept. Life Environ. Sci. Shimane Univ.

• Masumoto Ikuko

  Department Of Life And Environmental Sciences Shimane University

• Shigeoka Shigeru

  Department Of Advanced Bioscience Faculty Of Agriculture Kinki University

• Sawa Yoshihiro

  Department Of Life Science And Biotechnology Shimane University

• Nishikawa Hitoshi

  Department Of Life And Environmental Sciences Shimane University

• Nishikawa Hitoshi

  Department Of Chest Surgery Kitakyushu Municipal Medical Center

• Sawa Yoshihiro

  Dept. Appl. Biosci. And Biotech. Fac. Life And Environ. Sci. Shimane Univ.

• Shigeoka Shigeru

  Dept. Adv. Biosci. Fac. Agr. Kinki Univ

• Nakamura Ayana

  Department Of Advanced Bioscience Faculty Of Agriculture Kinki University

• Sakamoto Yoko

  Department Of Food Science And Technology College Of Bioresouce Sciences Nihon University

• Ishikawa Takahiro

  Department Of Clinical Cell Biology And Medicine Chiba University Graduate School Of Medicine

• Shibata Hitoshi

  Department Of Applied Biological Science Faculty Of Agriculture Shimane University

• Sawa Yoshihiro

  Department Of Life Science And Biotechnology Faculty Of Life And Environmental Science Shimane Unive

• Sawa Yoshihiro

  Department Of Applied Bioscience And Biotechnology Faculty Of Life And Environmental Science Shimane University

• Ishikawa Takahiro

  Department of Applied Bioscience and Biotechnology, Faculty of Life and Environmental Science, Shimane University

• SHIBATA Hitoshi

  Department of Agricultural Chemistry, Faculty of Agriculture, Shinshu University

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