Evaluation of the Defense System in Chloroplasts to Photooxidative Stress Caused by Paraquat Using Transgenic Tobacco Plants Expressing Catalase from Escherichia coli

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概要

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• We evaluated the defense system in chloroplasts to photooxidative stress imposed by paraquat treatment under illumination in transgenic tobacco plants with increased tolerance to drought stress at a high light intensity produced by catalase from Escherichia coli targeted to chloroplasts [Shikanai et al.(1998)FEBS Lett.428: 47]. At 24h after the paraquat application, Chl was destroyed in the wild-type plants, but not in transgenic plants. Photosynthetic activities monitored by CO_2 fixation and Chl fluorescence were much less affected by the paraquat treatment in transgenic lines. The activities of chloroplastic ascorbate peroxidase(APX) isoenzymes decreased in parallel with the depletion of ascorbate(AsA) in leaves in both lines. Paraquat treatment had no effect on the transcript level of chloroplastic APX isoenzymes, while it significantly lowered the level of their proteins. These data suggest that the depletion of AsA in chloroplasts under severe stress conditions inactivates and degrades chloroplastic APX isoenzymes.

• 日本植物生理学会の論文

著者

• Sano Satoshi

  Research Institute Of Innovative Technology For The Earth (rite):(present Address)faculty Of Biologi

• Miyagawa Y

  Multifunctional Material Science Department National Industrial Research Institute Of Nagoya

• Maruta Takanori

  Dep. Of Advanced Bioscience Fac. Of Agriculture Kinki Univ.

• Tamoi Masahiro

  Department Of Advanced Bioscience Faculty Of Agriculture Kinki University

• Shigeoka Shigeru

  Department Of Advanced Bioscience Faculty Of Agriculture Kinki University

• Shigeoka Shigeru

  Dept. Adv. Biosci. Fac. Agr. Kinki Univ

• Maruta Takanori

  Department Of Food And Nutrition Faculty Of Agriculture Kinki University

• Miyagawa Yoshiko

  Department Of Advanced Bioscience Faculty Of Agriculture Kinki University

• MIYAGAWA Yoshiko

  Graduate School of Advanced Life Science, Dept. Food and Nutr., Kinki Univ.

• SHIGEOKA Shigeru

  Graduate School of Advanced Life Science, Dept. Food and Nutr., Kinki Univ.

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