Expression Analysis of the VTC2 and VTC5 Genes Encoding GDP-L-Galactose Phosphorylase, an Enzyme Involved in Ascorbate Biosynthesis, in Arabidopsis thaliana

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概要

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• Arabidopsis thaliana contains two GDP-L-galactose phosphorylase genes, VTC2 and VTC5, which are critical for ascorbate (AsA) biosynthesis. We investigated the expression levels of both VTC2 and VTC5 genes in wild-type A. thaliana and the AsA deficient mutants during early seedling growth. Ascorbate accumulated to an equal extent in all genotypes up to 5 d post-germination (DPG). The transcript level of VTC2 was dominant, and increased in parallel with AsA accumulation in the wild type. On the other hand, the expression of VTC5 compensated for the reduced VTC2 transcription levels in the AsA deficient mutant vtc2-1 in young seedlings. A luciferase activity assay indicated that the VTC5 promoter was more active in young (2 DPG) cotyledons and that the VTC2 and VTC5 promoters drove a day-to-night variation in expression. The present work provides clues to the precise roles of VTC2 and VTC5 in AsA biosynthesis in A. thaliana at the young seedling stage.

• 社団法人 日本農芸化学会の論文

著者

• Shibata Hiroyuki

  Institute For Chemical Research Kyoto University

• Gao Yongshun

  Dept. Life Environ. Sci. Shimane Univ.

• Shigeoka Shigeru

  Department Of Advanced Bioscience Faculty Of Agriculture Kinki University

• Sawa Yoshihiro

  Department Of Life Science And Biotechnology Shimane University

• Maruta Takanori

  Department Of Food And Nutrition Faculty Of Agriculture Kinki University

• Ishikawa Takahiro

  Department Of Clinical Cell Biology And Medicine Chiba University Graduate School Of Medicine

• Shibata Hitoshi

  Department Of Applied Biological Science Faculty Of Agriculture Shimane University

• GAO Yongshun

  Department of Applied Bioscience and Biotechnology, Faculty of Life and Environmental Science, Shima

• BADEJO Adebanjo

  Department of Applied Bioscience and Biotechnology, Faculty of Life and Environmental Science, Shima

• PAGE Mike

  Biosciences, College of Life and Environmental Sciences, University of Exeter

• SMIRNOFF Nicholas

  Biosciences, College of Life and Environmental Sciences, University of Exeter

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