Cloning,Structural Analysis and Expression of the Gene Encoding Aspartate Aminotransferase from the Thermophilic Cyanobacterium Phormidium lapideum
スポンサーリンク
概要
- 論文の詳細を見る
The aspartate aminotransferase gene from the thermophilic cyanobacterium Phormidium lapideum was cloned and expressed in Escherichia coli. The ORF of 1167 nucleotides encodes a protein of 388 amino acids having a molecular weight of 42,099. A molecular model of PlAspAT shows structural features similar to those of the Thermits thermophilm AspAT.
- 社団法人日本生物工学会の論文
- 2003-04-25
著者
-
SHIBATA Hitoshi
Department of Life Science and Biotechnology, Faculty of Life and Environmental Science, Shimane Uni
-
Shibata Hiroyuki
Institute For Chemical Research Kyoto University
-
Ashida Hiroyuki
Research Institute of Molecular Genetics, Shimane University
-
Ashida Hiroyuki
Mol. Gen. Res. Inst. Shimane Univ.
-
Ashida Hiroyuki
Institute For Molecular Genetics Shimane University
-
Kim Hyeung
Department of Life Science and Biotechnology, Faculty of Life and Environmental Science, Shimane Uni
-
SAWA Yoshihiro
Department of Life Science and Biotechnology, Faculty of Life and Environmental Science, Shimane Uni
-
Shibata H
Dept. Life Environ. Sci. Shimane Univ.
-
Sawa Yoshihiro
Dept. Life Environ. Sci. Shimane Univ.
-
HAMADA Kensaku
Department of Information Science, Faculty of Science, Shimane University
-
Hamada Kensaku
Department Of Mathematics And Computer Science Faculty Of Science And Engineering Shimane University
-
Sawa Yoshihiro
Department Of Life Science And Biotechnology Shimane University
-
NAKAOKA MASAKI
Department of Life Science and Biotechnology,Faculty of Life and Environmental Science,Shimane Unive
-
YAGI MAYUMl
Department of Life Science and Biotechnology,Faculty of Life and Environmental Science,Shimane Unive
-
YAGI Mayumi
Department of Life Science and Biotechnology, Faculty of Life and Environmental Science, Shimane Uni
-
Yagi Mayuml
Department Of Life Science And Biotechnology Faculty Of Life And Environmental Science Shimane Unive
-
Hamada Kensaku
Department Of Information Science Faculty Of Science Shimane University
-
Nakaoka Masaki
Department Of Life Science And Biotechnology Faculty Of Life And Environmental Science Shimane Unive
-
Sakamoto Yoko
Department Of Food Science And Technology College Of Bioresouce Sciences Nihon University
-
Shibata Hitoshi
Department Of Applied Biological Science Faculty Of Agriculture Shimane University
-
Sawa Yoshihiro
Department Of Life Science And Biotechnology Faculty Of Life And Environmental Science Shimane Unive
-
Ashida Hiroyuki
Department Of Molecular And Functional Genomics Center For Integrated Research In Science Shimane Un
-
Sawa Yoshihiro
Department Of Applied Bioscience And Biotechnology Faculty Of Life And Environmental Science Shimane University
-
SHIBATA Hitoshi
Department of Agricultural Chemistry, Faculty of Agriculture, Shinshu University
-
Kim Hyeung
Department of Life Science and Biotechnology,Faculty of Life and Environmental Science,Shimane University
-
Hamada Kensaku
Department of Chemistry, Faculty of Science, Shimane University
関連論文
- High Thermal Conductivity of Gallium Nitride (GaN) Crystals Grown by HVPE Process
- Catalase Catalyzes of Peroxynitrite-mediated Phenolic Nitration
- Electrical Resistivity Measurements of Pd_Cu_Ni_P_ Alloy in Supercooled Liquid and Liquid State
- Thermal Diffusivities and Conductivities of Molten Germanium and Silicon
- Measurement of Thermal Diffusivity of Steels at Elevated Temperature by a Laser Flash Method
- Applicability of Platinum and Molybdenum Coatings for Measuring Thermal Diffusivity of Transparent Glass Specimens by the Laser Flash Method at High Temperatures
- Expression of a Gene for Cyclophilin Which Contains an Amino-Terminal Endoplasmic Reticulum-Targeting Signal
- Natural Antioxidant, Chlorogenic Acid, Protects Against DNA Breakage Caused by Monochloramine
- In-situ Observation of Intragranular Ferrite Nucleation at Oxide Particles
- Altering the Substrate Specificity of Glutamate Dehydrogenase from Bacillus subtilis by Site-Directed Mutagenesis
- Two Chemotypes of Boletinus cavipes
- Volemolide, a Novel Norsterol from the Fungus Lactarius volemus
- Cloning and Sequence Analysis of Genes for Cyclophilin from Arabidopsis thaliana : GENE STRUCTURE AND EXPRESSION
- Molecular Properties and Enhancement of Thermostability by Random Mutagenesis of Glutamate Dehydrogenase from Bacillus subtilis
- Iron Chelation by Chlorogenic Acid as a Natural Antioxidant
- 2-III-22 Intermolecular dimmer structure of Euglena ascorbate peroxidase
- Functional Characterization of D-Galacturonic Acid Reductase, a Key Enzyme of the Ascorbate Biosynthesis Pathway, from Euglena gracilis
- Characterization of Protein Phosphatase 2A Acting on Phosphorylated Plasma Membrane Aquaporin of Tulip Petals
- Phosphorylation of Plasma Membrane Aquaporin Regulates Temperature-Dependent Opening of Tulip Petals
- Generation of Reactive Oxygen Species from Hinokitiol under Near-UV Irradiation(Analytical Chemistry)
- Molecular Properties and Activity of Amino-Terminal Truncated Forms of Lipase Activator Protein
- Calcium Ion-Dependent Reactivation of a Pseudomonas Lipase by Its Specific Modulating Protein, LipB^1
- Analysis of Dephosphorization Reaction Using a Simulation Model of Hot Metal Dephosphorization by Multiphase Slag
- Structure and Replication of Cryptic Plasmids, pMA1 and pMA2, from a Unicellular Cyanobacterium, Microcystis aeruginosa
- Replication of Filamentous Cyanobacterial Plasmids, pPF1 from Phormidium foveolarum and pPB1 from Plectonema boryanum
- Characterization of a Small Cryptic Plasmid, pPF1; from Phormidium foveolarum and Vector Construction
- Structural Organization of a Cryptic Plasmid, pMA1, from Microcystis aeruginosa f. aeruginosa Kutzing
- Simultaneous Control of Regio- and Stereoselectivity in the Reaction of Pentadienyltin by the Lewis Acid-Coordination to Its Ethereal Functionality
- Modeling the Behavior of Al_2O_3 Inclusions during the Dendritic Solidification of Steel
- Distribution of P_2O_5 between Solid Dicalcium Silicate and Liquid Phases in CaO-SiO_2-Fe_2O_3 System
- Mass Transfer of P_2O_5 between Liquid Slag and Solid Solution of 2CaO・SiO_2 and 3CaO・P_2O_5
- Origin of Heat Transfer Anomaly and Solidifying Shell Deformation of Peritectic Steels in Continuous Casting
- Recovery of Hot Ductility by Improving Thermal Pattern of Continuously Cast Low Carbon and Ultra Low Carbon Steel Slabs for Hot Direct Rolling
- Two Cytosolic Cyclophilin Genes of Arabidopsis thaliana Differently Regulated in Temporal-and Organ-Specific Expression
- Distribution of P_2O_5 between Solid Solution of 2CaO・SiO_2-3CaO・P_2O_5 and Liquid Phase
- Precipitation Behavior of Copper, Tin and Manganese Sulfide at High Temperature in Fe-10%Cu-0.5%Sn Alloys
- Cysteine Uptake for Accumulation of Glutathione by the Cyanobacterium Synechocystis strain PCC 6803
- CLONING OF PROLYL ISOMERASE GENES FROM ARABIDOPSIS THALIANA
- Repraesentins D, E and F, New Plant Growth Promoters from Lactarius repraesentaneus
- Anti-inflammatory Cyathane Diterpenoids from Sarcodon scabrosus
- New Plant Growth Promoters, Repraesentins A, B and C, from Lactarius repraesentaneus(Organic Chemistry)
- Properties of Lipid-soluble Vitamins in Egg Albumin Complexes(Food & Nutrition)
- Preparation of Water-soluble and Stable Chlorophylls and β-Carotene by Forming Complexes with Egg Albumin(Food & Nutrition)
- Near-UV Irradiation Induces Shock Proteins in Anacystis nidulans R-2; Possible Role of Active Oxygen
- Asiaticusin A and B, Novel Prenylated Phenolics from Boletinus asiaticus and B. paluster (Boletaceae) Fungi
- Hydroxyl Radical Generation Depending on O_2 or H_2O by a Photocatalyzed Reaction in an Aqueous Suspension of Titanium Dioxide
- Oxidative Depolymerization of Chitosan by Hydroxyl Radical
- A High Activity of 3-Hydroxy-3-methylglutaryl Coenzyme A Reductase in Chloroplasts of Stevia rebaudiana Bertoni
- Determination of Free Energy of δ-Ferrite/γ-Austenite Interphase Boundary of Low Carbon Steels by In-situ Observation
- In-situ Observation of Engulfment and Pushing of Nonmetallic Inclusions in Steel Melt by Advancing Melt/Solid Interface
- Characteristics of Agglomeration of Various Inclusion Particles on Molten Steel Surface
- "In-situ"Observation of Collision, Agglomeration and Cluster Formation of Alumina Inclusion Particles on Steel Melts
- Characterization of Four Plasma Membrane Aquaporins in Tulip Petals : A Putative Homolog is Regulated by Phosphorylation
- Cloning, Biochemical and Phylogenetic Characterizations of γ-Glutamylcysteine Synthetase from Anabaena sp. PCC 7120
- Cloning,Structural Analysis and Expression of the Gene Encoding Aspartate Aminotransferase from the Thermophilic Cyanobacterium Phormidium lapideum
- Characterization of Aspartate Aminotransferase from the Cyanobacterium Phormidium lapideum(Biochemistry & Molecular Biology)
- Oxidation and Reduction of Nitrite Ion in the TiO_2 Photo-induced Catalytic Reaction
- Effect of Intracellular Glutathione on Heat-induced Cell Death in the Cyanobacterium, Synechocystis PCC 6803
- Heat Transfer across Mold Flux Film in Mold during Initial Solidification in Continuous Casting of Steel
- Thermal Resistance at the Interface between Mold Flux Film and Mold for Continuous Casting of Steels
- Radiative Heat Transfer through Mold Flux Film during Initial Solidification in Continuous Casting of Steel
- Heat Transfer from Shell across Flux Film to Continuous Casting Mold
- Measurement of Optical Properties of Continuous Casting Mold Fluxes at Elevated Temperatures
- Thermal Resistance between Solidifying Steel Shell and Continuous Casting Mold with Intervening Flux Film
- Anomaly in Melting Behavior and Mechanical Properties of Carbon Steels Near Their Solidus Temperature
- "In-situ"Real Time Observation of Planar to Cellular and Cellular to Dendritic Transition of Crystals Growing in Fe-C Alloy Melts
- REGULATION OF PROLYL ISOMERASE GENE EXPRESSION IN ARABIDOPSIS
- CHARACTERIZATION OF CYCLOPHILIN FROM ARABIDOPSIS THALIANA
- Chemical State of Chromium in CaO-SiO_2 Base Oxides Annealed under Different Conditions
- EXPRESSION OF LUCIFERASE GENES INCYANOBACTERIUM PHORMIDIUM LAPIDEUM
- CLONING AND EXPRESSION ANALYSIS OFCYCLOPHLIN GENES FROM ARABIDOPSIS THALIANA
- PCR CLONING OF THE GLUTAMINE SYNTHETASEGENE FROM A THRMOPHILIC CYANOBACTERIUM
- 1-III-3 Vitamin C Biosynthesis and Translocation in Tomato (Lycopersicum esculentum) Fruits
- Chloroplast Development in 4-Thiouridine-Cultured Radish Seedlings VI. : Anabolic Pathway of 4-Thiouridine
- A Non-NadB Type L-Aspartate Dehydrogenase from Ralstonia eutropha Strain JMP134: Molecular Characterization and Physiological Functions
- Influence of Bottom Bubbling Condition on Metal Emulsion Formation in Lead-Salt System
- Changes in the IAA Metabolic Content of Zea mays L Kernels during Maturation(Organic Chemistry)
- Isolation of Zeanoside C, a New Metabolite of IAA from Corn (Zea mays L.) Kernels(Organic Chemistry)
- Isolation of Two New IAA Conjugates, 7'-O-β-D-Glucopyranoside of 3,7-Dihydroxy-2-Indolinone-3-Acetic Acid and 8'-O-β-D-Glucopyranoside of 8-Hydroxy-2-Quinolone-4-Carboxylic Acid, from Immature Sweet Corn Kernels(Zea mays L)(Organic Chemistry)
- Degree of Undercooling and Contact Angle of Pure Iron at 1933K on Single-crystal Al_2O_3, MgO, and MgAl_2O_4 under Argon Atmosphere with Controlled Oxygen Partial Pressure
- Evaluation of Bubble Eye Area to Improve Gas/Liquid Reaction Rates at Bath Surfaces
- Expression Analysis of the VTC2 and VTC5 Genes Encoding GDP-L-Galactose Phosphorylase, an Enzyme Involved in Ascorbate Biosynthesis, in Arabidopsis thaliana
- A Non-NadB Type L-Aspartate Dehydrogenase from Ralstonia eutropha Strain JMP134 : Molecular Characterization and Physiological Functions
- Expression Analysis of the VTC2 and VTC5 Genes Encoding GDP-L-Galactose Phosphorylase, an Enzyme Involved in Ascorbate Biosynthesis, in Arabidopsis thaliana
- Analysis of Two L-Galactono-1,4-Lactone-Responsive Genes with Complementary Expression During the Development of Arabidopsis thaliana
- Properties of α-Tocopherols Incorporated into Water-soluble Complexes(Food & Nutrition)
- TREATMENT FOR PULMONARY METASTASIS ARISING FROM OSTEOGENIC SARCOMA
- Crystal and molecular structure of the dicobalt(III) complex containing bridging di-.MU.-hydroxo ligands, [(gly)(en)Co(.MU.-OH)2Co(gly)(en)]2+.