Altering the Substrate Specificity of Glutamate Dehydrogenase from Bacillus subtilis by Site-Directed Mutagenesis
スポンサーリンク
概要
- 論文の詳細を見る
The Lys80, Gly82 and Met101 residues of glutamate dehydrogenase from Bacillus subtilis were mutated into a series of single mutants. The wild-type enzyme was highly specific for 2-oxoglutarate, whereas G82K and M101S dramatically switched to increased specificity for oxaloacetate with kcat values 3.45 and 5.68 s−1, which were 265-fold and 473-fold higher respectively than those for 2-oxoglutarate.
- 社団法人 日本農芸化学会の論文
著者
-
Khan Md.
Department Of Biosystems Engineering University Of Manitoba
-
Khan Md.
Department Of Environmental Management Engineering Faculty Of Environmental Science And Technology O
-
SHIBATA Hitoshi
Department of Life Science and Biotechnology, Faculty of Life and Environmental Science, Shimane Uni
-
Ashida Hiroyuki
Mol. Gen. Res. Inst. Shimane Univ.
-
Ashida Hiroyuki
Institute For Molecular Genetics Shimane University
-
Kim Hyeung
Department of Life Science and Biotechnology, Faculty of Life and Environmental Science, Shimane Uni
-
Ashida Hiroyuki
Department of Molecular and Functional Genomics, Center for Integrated Research in Science, Shimane
-
ISHIKAWA Takahiro
Department of Life Science and Biotechnology, Faculty of Life and Environmental Science, Shimane Uni
-
SAWA Yoshihiro
Department of Life Science and Biotechnology, Faculty of Life and Environmental Science, Shimane Uni
-
Sawa Yoshihiro
Department Of Life Science And Biotechnology Shimane University
-
Ishikawa Takahiro
Department Of Clinical Cell Biology And Medicine Chiba University Graduate School Of Medicine
-
Shibata Hitoshi
Department Of Applied Biological Science Faculty Of Agriculture Shimane University
-
Khan Md.
Department Of Animal Nutrition Bangladesh Agricultural University
-
Sawa Yoshihiro
Department Of Life Science And Biotechnology Faculty Of Life And Environmental Science Shimane Unive
-
Ashida Hiroyuki
Department Of Molecular And Functional Genomics Center For Integrated Research In Science Shimane Un
-
Sawa Yoshihiro
Department Of Applied Bioscience And Biotechnology Faculty Of Life And Environmental Science Shimane University
-
KHAN Md.
Department of Life Science and Biotechnology, Faculty of Life and Environmental Science, Shimane University
-
Ishikawa Takahiro
Department of Applied Bioscience and Biotechnology, Faculty of Life and Environmental Science, Shimane University
-
SHIBATA Hitoshi
Department of Agricultural Chemistry, Faculty of Agriculture, Shinshu University
-
ISHIKAWA Takahiro
Department of Life Science and Biotechnology, Faculty of Life and Environmental Science, Shimane University
-
SAWA Yoshihiro
Department of Life Science and Biotechnology, Faculty of Life and Environmental Science, Shimane University
-
Ashida Hiroyuki
Department of Molecular and Functional Genomics, Center for Integrated Research in Science, Shimane University
-
SHIBATA Hitoshi
Department of Life Science and Biotechnology, Faculty of Life and Environmental Science, Shimane University
-
KIM Hyeung
Department of Life Science and Biotechnology, Faculty of Life and Environmental Science, Shimane University
関連論文
- Catalase Catalyzes of Peroxynitrite-mediated Phenolic Nitration
- 3D analysis of the spinning behavior of flossy cocoon mutants in the silkworm, Bombyx mori
- Expression of a Gene for Cyclophilin Which Contains an Amino-Terminal Endoplasmic Reticulum-Targeting Signal
- Natural Antioxidant, Chlorogenic Acid, Protects Against DNA Breakage Caused by Monochloramine
- Altering the Substrate Specificity of Glutamate Dehydrogenase from Bacillus subtilis by Site-Directed Mutagenesis
- Cloning and Sequence Analysis of Genes for Cyclophilin from Arabidopsis thaliana : GENE STRUCTURE AND EXPRESSION
- Molecular Characterization and Physiological Role of a Glyoxysome-Bound Ascorbate Peroxidase from Spinach
- Molecular Properties and Enhancement of Thermostability by Random Mutagenesis of Glutamate Dehydrogenase from Bacillus subtilis
- Iron Chelation by Chlorogenic Acid as a Natural Antioxidant
- Functional Characterization of D-Galacturonic Acid Reductase, a Key Enzyme of the Ascorbate Biosynthesis Pathway, from Euglena gracilis
- Generation of Reactive Oxygen Species from Hinokitiol under Near-UV Irradiation(Analytical Chemistry)
- The Relationship Between a Leaf-rolling Moth (Dactylioglypha tonica) and Fungi Covering the Cocoon(Microbiology & Fermentation Technology)
- An Antibiotic from Penicillium sp. Covering the Cocoon of the Leaf-rolling Moth, Dactylioglypha tonica
- Structure and Replication of Cryptic Plasmids, pMA1 and pMA2, from a Unicellular Cyanobacterium, Microcystis aeruginosa
- Replication of Filamentous Cyanobacterial Plasmids, pPF1 from Phormidium foveolarum and pPB1 from Plectonema boryanum
- Structural Organization of a Cryptic Plasmid, pMA1, from Microcystis aeruginosa f. aeruginosa Kutzing
- Uptake of CDDP-containing Polymeric Micelles by Cells Using Particle Induced X-Ray Emission
- Two Cytosolic Cyclophilin Genes of Arabidopsis thaliana Differently Regulated in Temporal-and Organ-Specific Expression
- Cysteine Uptake for Accumulation of Glutathione by the Cyanobacterium Synechocystis strain PCC 6803
- CLONING OF PROLYL ISOMERASE GENES FROM ARABIDOPSIS THALIANA
- Properties of Lipid-soluble Vitamins in Egg Albumin Complexes(Food & Nutrition)
- Preparation of Water-soluble and Stable Chlorophylls and β-Carotene by Forming Complexes with Egg Albumin(Food & Nutrition)
- Near-UV Irradiation Induces Shock Proteins in Anacystis nidulans R-2; Possible Role of Active Oxygen
- Hydroxyl Radical Generation Depending on O_2 or H_2O by a Photocatalyzed Reaction in an Aqueous Suspension of Titanium Dioxide
- Oxidative Depolymerization of Chitosan by Hydroxyl Radical
- A High Activity of 3-Hydroxy-3-methylglutaryl Coenzyme A Reductase in Chloroplasts of Stevia rebaudiana Bertoni
- Characterization of Four Plasma Membrane Aquaporins in Tulip Petals : A Putative Homolog is Regulated by Phosphorylation
- Cloning, Biochemical and Phylogenetic Characterizations of γ-Glutamylcysteine Synthetase from Anabaena sp. PCC 7120
- Cloning,Structural Analysis and Expression of the Gene Encoding Aspartate Aminotransferase from the Thermophilic Cyanobacterium Phormidium lapideum
- Characterization of Aspartate Aminotransferase from the Cyanobacterium Phormidium lapideum(Biochemistry & Molecular Biology)
- Oxidation and Reduction of Nitrite Ion in the TiO_2 Photo-induced Catalytic Reaction
- Effect of Intracellular Glutathione on Heat-induced Cell Death in the Cyanobacterium, Synechocystis PCC 6803
- REGULATION OF PROLYL ISOMERASE GENE EXPRESSION IN ARABIDOPSIS
- CHARACTERIZATION OF CYCLOPHILIN FROM ARABIDOPSIS THALIANA
- EXPRESSION OF LUCIFERASE GENES INCYANOBACTERIUM PHORMIDIUM LAPIDEUM
- CLONING AND EXPRESSION ANALYSIS OFCYCLOPHLIN GENES FROM ARABIDOPSIS THALIANA
- PCR CLONING OF THE GLUTAMINE SYNTHETASEGENE FROM A THRMOPHILIC CYANOBACTERIUM
- Uptake of CDDP-containing Polymeric Micelles by Cells Using Particle Induced X-Ray Emission
- Cocoon repair behavior of Bombyx mori silkworm
- Responses of Semi-Scavenging F_1 Crossbred (Rhode Island Red Male × Fayoumi Female) Grower and Per-Layer Chickens to Diets of Different Nutrient Density Formulated with Locally Available Feed Ingredients
- A Non-NadB Type L-Aspartate Dehydrogenase from Ralstonia eutropha Strain JMP134: Molecular Characterization and Physiological Functions
- Primary lung cancer associated with Werner syndrome
- Subcellular Localization and Properties of L-Galactono-γ-lactone Dehydrogenase in Spinach Leaves
- Changes in the IAA Metabolic Content of Zea mays L Kernels during Maturation(Organic Chemistry)
- Isolation of Zeanoside C, a New Metabolite of IAA from Corn (Zea mays L.) Kernels(Organic Chemistry)
- Isolation of Two New IAA Conjugates, 7'-O-β-D-Glucopyranoside of 3,7-Dihydroxy-2-Indolinone-3-Acetic Acid and 8'-O-β-D-Glucopyranoside of 8-Hydroxy-2-Quinolone-4-Carboxylic Acid, from Immature Sweet Corn Kernels(Zea mays L)(Organic Chemistry)
- Recent Advances in Ascorbate Biosynthesis and the Physiological Significance of Ascorbate Peroxidase in Photosynthesizing Organisms
- Sound Intensity Measurement for Directional Property of Jet Screech (第25回可視化情報シンポジウム講演論文集)
- Expression Analysis of the VTC2 and VTC5 Genes Encoding GDP-L-Galactose Phosphorylase, an Enzyme Involved in Ascorbate Biosynthesis, in Arabidopsis thaliana
- Supplementation with Urea and Molasses and Body Weight, Milk Yield and Onset of Ovarian Cyclicity in Cows
- Fine Structure Analysis of Ramie Fivres by Small Angle X-Ray Scattering Technique
- A Non-NadB Type L-Aspartate Dehydrogenase from Ralstonia eutropha Strain JMP134 : Molecular Characterization and Physiological Functions
- Expression Analysis of the VTC2 and VTC5 Genes Encoding GDP-L-Galactose Phosphorylase, an Enzyme Involved in Ascorbate Biosynthesis, in Arabidopsis thaliana
- 国際ボランティア活動への徳島大学学生の参加 : 北海道大学歯学研究科主管 JICA 草の根支援事業 (バングラディシュ) 支援学生チームへの参加
- Effects of incubation times and moisture regimes on pH values and exchangeable cations in acid sulfate soils as influenced by application rates of basic slag
- Use of Duckweed (Lemna perpusilla) as a Protein Source Feed Item in the Diet of Semi-Scavenging Jinding Layer Ducks
- Analysis of Two L-Galactono-1,4-Lactone-Responsive Genes with Complementary Expression During the Development of Arabidopsis thaliana
- Properties of α-Tocopherols Incorporated into Water-soluble Complexes(Food & Nutrition)
- Theory of Radiation Force on Carbon Nanotubes
- TREATMENT FOR PULMONARY METASTASIS ARISING FROM OSTEOGENIC SARCOMA
- Activation of γ-Aminobutyrate Production by Chloroplastic H_2O_2 Is Associated with the Oxidative Stress Response