Characterization of Aspartate Aminotransferase from the Cyanobacterium Phormidium lapideum(Biochemistry & Molecular Biology)
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概要
- 論文の詳細を見る
Aspartate aminotransferase (AspAT) was purified to homogeneity from cell extracts of the non-N_2-fixing cyanobacterium Phormidium lapideum. The NH_2-terminal sequence of 25 amino acid residues was different from the sequences of the subfamily Iα of AspATs from eukaryotes and Escherichia coli, but it was similar to sequences of the subfamily Iγ of AspATs from archaebacteria and eubacteria. The enzyme was most active at 80℃ and was stable at up to 75℃. Thermal inactivation (60-85℃) of the enzyme followed first-order kinetics, with 2-oxoglutarate causing a shift of the thermal inactivation curves to higher temperatures. However, at 25℃ the k_<cat> of P. lapideum AspAT was nearly equal to the values of AspATs from mesophilic organisms. The enzyme used L-aspartate and L-cysteine sulfinate as amino donors and 2-oxoglutarate as an amino acceptor. The K_m values were 5.0 mM for L-aspartate, 5.7 mM for L-glutamate, 0.2 mM for 2-oxoglutarate, and 0.032 mM for oxaloacetate.
- 社団法人日本農芸化学会の論文
- 2003-03-23
著者
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SHIBATA Hitoshi
Department of Life Science and Biotechnology, Faculty of Life and Environmental Science, Shimane Uni
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IKEGAMI Koji
Department of Molecular Anatomy, Hamamatsu University School of Medicine
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Shibata Hiroyuki
Institute For Chemical Research Kyoto University
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Kim Hyeung
Department of Life Science and Biotechnology, Faculty of Life and Environmental Science, Shimane Uni
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SAWA Yoshihiro
Department of Life Science and Biotechnology, Faculty of Life and Environmental Science, Shimane Uni
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Ikegami Koji
Department Of Life Science And Biotechnology Faculty Of Life And Environmental Science Shimane Unive
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Shibata H
Dept. Life Environ. Sci. Shimane Univ.
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Sawa Yoshihiro
Dept. Life Environ. Sci. Shimane Univ.
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Sawa Yoshihiro
Department Of Life Science And Biotechnology Shimane University
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NAKAOKA MASAKI
Department of Life Science and Biotechnology,Faculty of Life and Environmental Science,Shimane Unive
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YAGI Mayumi
Department of Life Science and Biotechnology, Faculty of Life and Environmental Science, Shimane Uni
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Yagi Mayuml
Department Of Life Science And Biotechnology Faculty Of Life And Environmental Science Shimane Unive
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Sawa Yoshihiro
Dept. Appl. Biosci. And Biotech. Fac. Life And Environ. Sci. Shimane Univ.
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Nakaoka Masaki
Department Of Life Science And Biotechnology Faculty Of Life And Environmental Science Shimane Unive
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Sakamoto Yoko
Department Of Food Science And Technology College Of Bioresouce Sciences Nihon University
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Shibata Hitoshi
Department Of Applied Biological Science Faculty Of Agriculture Shimane University
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Sawa Yoshihiro
Department Of Life Science And Biotechnology Faculty Of Life And Environmental Science Shimane Unive
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Sawa Yoshihiro
Department Of Applied Bioscience And Biotechnology Faculty Of Life And Environmental Science Shimane University
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SHIBATA Hitoshi
Department of Agricultural Chemistry, Faculty of Agriculture, Shinshu University
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Ikegami Koji
Department of Cell Biology and Anatomy, Hamamatsu University School of Medicine
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Kim Hyeung
Department of Life Science and Biotechnology,Faculty of Life and Environmental Science,Shimane University
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