Chloroplast Development in 4-Thiouridine-Cultured Radish Seedlings VI. : Anabolic Pathway of 4-Thiouridine
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概要
- 論文の詳細を見る
In germinating radish seeds, [U-^<14>C]-4-thiouridine was converted to 4-thio-UMP, 4-thio-UDP, 4-thio-UTP, 4-thio-UDP glucose and 4-thiouracil, of which 4-thiouracil accounted for 60-85%. 4-Thio-UTP is incorporated into RNAs of radish seedlings [Shibata et al. (1980) FEBS Lett. 119: 85]. These same metabolites were labeled following germination of radish seeds with [2-^<14>C]-4-thiouracil. 4-Thiouridine was hydrolyzed by the uridine nucleosidase (EC 3.2.2.3) of radish seedlings as effectively as was uridine. The activity of uridine nucleosidase was increased by germination with 4-thiouridine. These results are a strong indication that 4-thiouridine is converted to 4-thiouracil, then to 4-thio-UMP by uracil phosphoribosyltransferase (EC 2.4.2.9). The alternative formation of 4-thio-UMP from 4-thiouridine by uridine kinase (EC 2.7.1.48) also was suggested. A possible mechanism which may cause inhibition of chloroplast biogenesis in 4-thiouridine-cultured seedlings is discussed.
- 日本植物生理学会の論文
著者
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Ochiai Hideo
Dept. Appl. Biosci. Fac. Agr. Shimaneuniv.
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Ochiai Hideo
Laboratory Of Biochemistry College Of Agriculture Shimane University
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Shibata H
Dept. Life Environ. Sci. Shimane Univ.
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Sawa Yoshihiro
Dept. Life Environ. Sci. Shimane Univ.
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Omura H
Department Of Biomolecular Science Gifu University:ikeda Food Research Co. Ltd.
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Sawa Yoshihiro
Laboratory of Applied Biological Science, Faculty of Agriculture
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SHIBATA Hitoshi
Laboratory of Biochemistry, College of Agriculture, Shimane University
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Fujiwara Toshinori
Laboratory of Biochemistry, College of Agriculture, Shimane University
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Fujiwara Toshinori
Laboratory Of Biochemistry College Of Agriculture Shimane University
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Shibata Hitoshi
Laboratory Of Biochemistry College Of Agricidture Shimane University
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Ochiai Hideo
Laboratory Of Applied Biological Science Faculty Of Agriculture Shimane University
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