Structure and Replication of Cryptic Plasmids, pMA1 and pMA2, from a Unicellular Cyanobacterium, Microcystis aeruginosa
スポンサーリンク
概要
- 論文の詳細を見る
The 4993-bp cryptic plasmid, pMA2, from a cyanobacterium, Microcystis aeruginosa f. aeruginosa Kutzing, originally derived from Kasumigaura lake, was completely sequenced and analyzed. Plasmid pMA2 had a unique sequence motif CTTGATT, which was proposed to be a nicking site on the smaller plasmid pMA1 (2287-bp) by the presence of single-stranded DNA susceptible to S1 nuclease. We had detected the occurrence of the single-stranded pMA1 in the living M. aeruginosa cells. This was also the case of pMA2. Thus, we suggest that both plasmids, pMA1 and pMA2, replicate through the rolling circle mechanism. By computer analysis of the pMA2 sequence, two open reading frames were found : one had a predicted molecular size of 30,440 Da and the other on a complementary one had a predicted molecular size of 10,852 Da. No rep protein was detected. Replication mechanisms of the plasmids are discussed.
- 社団法人日本農芸化学会の論文
- 1995-07-23
著者
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Ashida Hiroyuki
Research Institute of Molecular Genetics, Shimane University
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Ashida Hiroyuki
Mol. Gen. Res. Inst. Shimane Univ.
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Ashida Hiroyuki
Institute For Molecular Genetics Shimane University
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Ochiai Hideo
Dept. Appl. Biosci. Fac. Agr. Shimaneuniv.
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Ochiai Hideo
Laboratory Of Biochemistry College Of Agriculture Shimane University
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Sawa Yoshihiro
Dept. Life Environ. Sci. Shimane Univ.
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Omura H
Department Of Biomolecular Science Gifu University:ikeda Food Research Co. Ltd.
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Tominaga Hiroshi
Laboratory Of Applied Biological Science Faculty Of Agriculture
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Kawagishi Shouichi
Laboratory of Applied Biological Science, Faculty of Agriculture
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Sawa Yoshihiro
Laboratory of Applied Biological Science, Faculty of Agriculture
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Kawagishi Shouichi
Laboratory Of Applied Biological Science Faculty Of Agriculture
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Sakamoto Yoko
Department Of Food Science And Technology College Of Bioresouce Sciences Nihon University
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Ashida Hiroyuki
Department Of Molecular And Functional Genomics Center For Integrated Research In Science Shimane Un
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Ochiai Hideo
Laboratory Of Applied Biological Science Faculty Of Agriculture Shimane University
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