Arabidopsis chimeric TCP3 repressor produces novel floral traits in Torenia fournieri and Chrysanthemum morifolium

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概要

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• Chimeric REpressor gene-Silencing Technology (CRES-T) is a powerful gene-silencing tool to analyze the function of Arabidopsis transcription factors. To investigate whether CRES-T is also applicable to horticultural plants inadequate for genetic engineering because of their limited molecular biological characterization and polyploidy, we applied CRES-T to torenia and the hexaploid chrysanthemum and produced their transgenic plants expressing the chimeric repressor derived from the Arabidopsis TEOSINTE BRANCHED1, CYCLOIDEA, and PCF family transcription factor 3 (TCP3) fused with a plant-specific transcriptional repression domain named SRDX, consisting of 12 amino acids originated from the EAR-motif (TCP3-SRDX). Transgenic torenia and chrysanthemum expressing TCP3-SRDX exhibited fringed leaves and short pistils, while those expressing TCP3 fused with either the mutated repression domain (TCP3-mSRDX) or the overexpressor of TCP3 (TCP3-ox) did not exhibit phenotypic changes. In addition to fringed leaves, TCP3-SRDX transgenic torenia plants exhibited petals with fringed margins, distinctive color patterns, and reduced anthocyanin accumulation. In TCP3-SRDX transgenic chrysanthemum plants, floral organ development was suppressed as compared with the wild type. These results indicate that the Arabidopsis-derived TCP3-SRDX induced morphological changes in transgenic torenia and chrysanthemum although the observed phenotypes partially differ from each other. CRES-T may function in various plant species including polyploid species and modify their biological characteristics.

• 2011-03-25

著者

• Shinozaki K

  Riken Plant Sci. Center Yokohama Jpn

• Shinozaki K

  Riken Ibaraki Jpn

• Shinozaki K

  Inst. Physical And Chemical Res. (riken) Ibaraki Jpn

• Aida Ryutaro

  National Institute Of Floricultural Science National Agricultural Research Organization

• Ohme Takagi

  Research Institute Of Genome-based Biofactory National Institute Of Advanced Industrial Science And

• SHINOZAKI Kazuo

  Plant Mol. Biol. RIKEN

• Soeno Kazuo

  RIKEN Plant Science Center

• SHIZOZAKI Kazuo

  Plant Mol. Biol. RIKEN

• SHINOZAKI Kazuo

  Inst Physic Chem Res (RIKEN)

• Nakayama Masayoshi

  National Institute of Floricultural Science, Fujimoto

• Yamaguchi Shinozaki

  Biological Resources Division Japan International Research Center For Agricultural Sciences (jircas)

• Shinozaki Kazuo

  Tsukuba Institute (RIKEN)

• HIGUCHI Yohei

  Graduate School of Life and Environmental Sciences, University of Tsukuba

• Shinozaki Kazuo

  Plant Mol. Biol. Lab.

• Shinozaki Kazuo

  Laboratory Of Plant Molecular Biology Tsukuba Life Center Riken (the Institute Of Physical And Chemi

• Nishihara Masahiro

  Iwate Biotechnology Research Center

• Shinozaki K

  Univ. Tsukuba Ibaraki Jpn

• SASAKI Katsutomo

  National Institute of Floricultural Science

• NARUMI Takako

  National Institute of Floricultural Science

• Higuchi Youhei

  Univ. Tsukuba Ibaraki Jpn

• Saitoh Kumi

  Yamagata General Agricultural Research Center Department Of Agro-production Science

• Hayashi Yoriko

  Riken Saitama Jpn

• Higuchi Youhei

  Gene Research Center Graduate School Of Life And Environmental Sciences University Of Tsukuba

• TSUKATANI Nobue

  Iwate Biotechnology Research Center

• Yamaguchi Shinozaki

  Laboratory Of Plant Molecular Physiology Graduate School Of Agricultural And Life Sciences Universit

• Shinozaki Kazuo

  Lab. Of Pmb Riken

• Sako Kaori

  Faculty Of Advanced Life Science Graduate School Of Life Science Hokkaido University

• Sage‐ono K

  Graduate School Of Life And Environmental Sciences Univ. Of Tsukuba

• Nakata Masaru

  Bioproduction Res. Inst. National Inst. Of Advanced Industrial Sci. And Technol. (aist)

• Ohme Takagi

  Research Institute Of Genome-based Biofactory National Institute Of Advanced Industrial Science And

• Shikata Masahito

  National Institute of Floricultural Science, National Agriculture and Food Research Organization (NA

• Yamaguchi Hiroyasu

  National Inst. Of Floricultural Sci. National Agriculture And Food Res. Organization (naro)

• Ohme Takagi

  Bioproduction Research Institute National Institute Of Advanced Industrial Science And Technology (a

• Koyama Tomotsugu

  Bioproduction Res. Inst. National Inst. Of Advanced Industrial Sci. And Technol. (aist)

• Ohtsubo Norihiro

  National Inst. Of Floricultural Sci. National Agriculture And Food Res. Organization (naro)

• Sage-ono Kimiyo

  Gene Res. Center Graduate School Of Life And Environmental Sciences Univ. Of Tsukuba

• Nishihara Masahiro

  Bioproduction Res. Inst. National Inst. Of Advanced Industrial Sci. And Technol. (aist)

• Shibata Michio

  National Institute Of Floricultural Science National Agriculture And Food Research Organization

• Shinozaki Kazuo

  Riken Plant Sci. Center Yokohama Jpn

• Aida Ryutaro

  National Institute Of Floricultural Science

• Ohme Takagi

  Bioproduction Research Institute National Institute Of Advanced Industrial Science And Technology

• Saito Kazuki

  Metabolomics Research Group Riken Plant Science Center

• Ohtsubo Norihiro

  National Inst. Of Floricultural Sci. (nifs) National Agriculture And Food Res. Organization (naro)

• Koyama Tomotsugu

  Bioproduction Research Institute National Institute Of Advanced Industrial Science And Technology (a

• Nakayama Masayoshi

  National Institute Of Floricultural Science

• Nakayama Masayoshi

  National Inst. Of Floricultural Sci. National Agriculture And Food Res. Organization

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