Molecular Cloning and Characterization of a cDNA Encoding Proline Transporter in Rice
スポンサーリンク
概要
- 論文の詳細を見る
A cDNA encoding a proline(Pro)transporter(ProT)was isolated and characterized from a cDNA library prepared from 14-d-old seedlings of Oryza sativa cv.Akibare. The deduced amino acid sequence of the rice ProT protein(OsProT)had 68.8% homology to the ProT protein 1 from Arabidopsis thaliana and 59.6% homology to that from Lycopersicon esculantum. Norhtern blot analysis revealed that the gene for OsProT(OsProT)was expressed in all organs examined, comparatively strongly in leaf sheath and stem. Salt treatment did not induce expression of OsProT but strongly induced expression of the gene for Δ^1-pyrroline-5-carboxylate synthetase(P5CS), a key enzyme in Pro biosynthesis. Southern blot analysis revealed that OsProT has a gene family. OsProT specifically transported L-Pro in a transport assay using Xenopus laevis oocytes.
- 日本植物生理学会の論文
著者
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SHINOZAKI Kazuo
Laboratory of Plant Molecular Biology, RIKEN Tsukuba Institute
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Shinozaki K
Riken Plant Sci. Center Yokohama Jpn
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Shinozaki K
Inst. Physical And Chemical Res. (riken) Ibaraki Jpn
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IGARASHI Yumiko
Life Science Group, Hitachi, Ltd.
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Igarashi Yumiko
Life Science Group Hitachi Ltd.
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Igarashi Yumiko
Central Research Laboratory, Hitachi, Ltd.
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Yoshiba Yoshu
Central Research Laboratory, Hitachi, Ltd.
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Takeshita Tomoko
Central Research Laboratory, Hitachi, Ltd.
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Nomura Sayuri
Central Research Laboratory, Hitachi, Ltd.
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Otomo Jun
Central Research Laboratory, Hitachi, Ltd.
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Yamaguchi-Shinozaki Kazuko
Biological Resources Division, Japan International Research Center for Agricultural Sciences(JIRCAS)
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SHIZOZAKI Kazuo
Plant Mol. Biol. RIKEN
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SHINOZAKI Kazuo
Inst Physic Chem Res (RIKEN)
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Yamaguchi‐shinozaki Kazuko
Univ. Tokyo Tokyo Jpn
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Yamaguchi‐shinozaki K
Japan International Res. Center For Agricultural Sci. (jircas) Ibaraki Jpn
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Yamaguchi-shinozaki Kazuko
Japan. Int. Res. Cent. Agri. Sci.
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Yamaguchi-shinozaki Kazuko
Biological Resources Division Japan International Research Center For Agicultural Sciences (jircas)
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Shinozaki Kazuo
Plant Mol. Biol. Lab.
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Yoshiba Yoshu
Cent. Res. Lab. Hitachi Ltd.
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Otomo Jun
Central Research Laboratory Hitachi Ltd.:(present Address)life Science Group Hitachi Ltd.
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Nomura Sayuri
Central Research Laboratory Hitachi Ltd.:(present Address)life Science Group Hitachi Ltd.
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Yoshida Yuhji
Cent. Res. Lab. Hitachi Ltd.
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Shinozaki Kazuo
Laboratory Of Plant Molecular Biology Riken
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Takeshita Tomoko
Central Research Laboratory Hitachi Ltd.:(present Address)life Science Group Hitachi Ltd.
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