Novel Drought-Inducible Genes in the Highly Drought-Tolerant Cowpea: Cloning of cDNAs and Analysis of the Expression of the Corresponding Genes
スポンサーリンク
概要
- 論文の詳細を見る
Ten cDNAs of genes that were induced by dehydration stress were cloned by differential screening from the highly drought-tolerant legume, cowpea (Vigna unguiculata), a major crop in West Africa. The clones were collectively named CPRD (cowpea clones responsive to dehydration). Northern blot analysis revealed that nine of the CPRD genes were induced by dehydration stress, but the timing of induction of mRNA synthesis varied among the CPRD genes. We analyzed the effects of other environmental stresses on the expression of the CPRD8, CPRD14 and CPRD22 genes, and we found that these genes were strongly induced by high-salinity stress but not by cold or heat stress. Drought-stressed cowpea plants accumulated abscisic acid (ABA) to a level that was 160 times higher than that in unstressed plants. The CPRD8 and CPRD22 genes were induced to a significant extent by the application of exogenous ABA but the CPRD14 gene was not. These results indicate the existence of at least two signal-transduction pathways between the detection of water stress and the expression of CPRD genes in cowpea. Sequence analysis of CPRD8 and CPRD22 cDNAs revealed that they encoded putative proteins that were related to old yellow enzyme and group 2 LEA proteins, respectively. The protein encoded by CPRD14 exhibited sequence homology to dihydroflavonol-4-reductase (DFR) and vesti-tone reductase (VR). Old yellow enzyme, DFR and VR have not been identified as drought-inducible proteins in other plants, whereas LEA genes have been well characterized as drought-inducible genes. The various gene products might function to protect cells from environmental stress.
- 日本植物生理学会の論文
著者
-
SHINOZAKI Kazuo
Laboratory of Plant Molecular Biology, RIKEN Tsukuba Institute
-
Yamaguchi-Shinozaki Kazuko
Biological Resources Division, Japan International Research Center for Agricultural Sciences(JIRCAS)
-
URAO Takeshi
Biological Resources Division, Japan International Research Center for Agricultural Sciences(JIRCAS)
-
IUCHI Satoshi
Laboratory of Plant Molecular Biology, Tsukuba Life Science Center, The Institute of Physical and Ch
-
Urao Takeshi
JIRCAS, Min. Agr. Forest Fish
-
Terao T
Japan International Res. Center For Agricultural Sci. Ministry Of Agriculture Forestry And Fisheries
-
Terao Tomio
Biological Resources Division Japan International Research Center For Agricultural Sciences(jircas)
-
Terao Tomio
Intl. Res. Center Agr. Sci. Tsukuba
-
Terao Tomio
Biological Resources Division Japan International Research Center For Agricultural Sciences
-
SHINOZAKI Kazuo
Inst Physic Chem Res (RIKEN)
-
Urao T
Jircas Min. Agr. Forest Fish
-
Urao Takeshi
Biological Resources Division Japan International Research Center For Agicultural Sciences (jircas)
-
Yamaguchi‐shinozaki Kazuko
Univ. Tokyo Tokyo Jpn
-
Yamaguchi‐shinozaki K
Japan International Res. Center For Agricultural Sci. (jircas) Ibaraki Jpn
-
Yamaguchi-shinozaki Kazuko
Japan. Int. Res. Cent. Agri. Sci.
-
Yamaguchi-shinozaki Kazuko
Biological Resources Division Japan International Research Center For Agicultural Sciences (jircas)
-
Iuchi Satoshi
Plant Mol. Biol. Lab.
-
Iuchi Satoshi
Biological Resource Center Riken Tsukuba Institute
-
Shinozaki Kazuo
Laboratory Of Plant Molecular Biology Riken
-
UMEZAWA Taishi
RIKEN Plant Science Center
-
Urao Takeshi
Plant Science Center Riken Yokohama Institute
-
Urao Takeshi
Riken Plant Science Center
-
Iuchi Satoshi
Laboratory Of Plant Molecular Biology The Institute Of Physical And Chemical Research (riken) Tsukub
関連論文
- Preparation of hydroxyapatite–ferrite composite particles by ultrasonic spray pyrolysis
- FUNCTIONAL ANALYSES OF AN Arabidopsis thaliana PHOSPHOLIPASE C-INTERACTING FACTOR
- A GENE ENCODING A PHOSPHATIDIC ACID PHOSPHATASE IS INDUCED BY HIGH-SALT AND LOW TEMPERATURE STRESSES IN Arabidopsis thaliana
- TWO-HYBRID cDNA CLONING AND FUNCTIONAL ANALYSES OF AN Arabidopsis PHOSPHOLIPASE C-INTERACTING PROTEIN
- Toxicity of Free Proline Revealed in an Arabidopsis T-DNA-Tagged Mutant Deficient in Proline Dehydrogenase
- Characterization of Full-length Enriched Expressed Sequence Tags of Stress-treated Poplar leaves
- RELATION BETWEEN THE LEVEL OF SALT TOLERANCE AND THE LEVEL OF Na^+ ABSORPTION IN PROLINE-PRETREATED RICE (Oryza sativa L.) UNDER SALT STRESS
- TISSUE-SPECIFICITY OF PROLINE ACCUMULATION AND CHANGES LEVELS OF FREE AMINO ACIDS IN SALT-STRESSED RICE(Oryza sativa L. )
- ROLES OF PROLINE IN OSMOTIC STRESS TOLERANCE AND MORPHOGENESIS OF Arabidopsis
- Characterization of the expression of the pene for △^1 -pyrroline-5-carboxylate synthetase and correlation between the expression of the gene and salt tolerance in Oryza sativa L.
- Molecular Cloning and Characterization of a cDNA Encoding Proline Transporter in Rice
- Increase by water stress treatment and tissue specific localization of polyamines in Arabidopsis thaliana
- Expression Profiles of Arabidopsis Phospholipase A IIA Gene in Response to Biotic and Abiotic Stresses
- Profiling gene expression patterns under drought, cold and salt stress using Arabidopsis full-length cDNA microarray
- MOLECULAR ANALYSIS OF NOVEL DROUGHT-AND COLD-STRESS-INDUCIBLE GENES ISOLATED BY FULL-LENGTH-cDNA MICRO ARRAY SYSTEM IN ARABIDOPSIS
- A Resource of 5,814 Dissociation Transposon-tagged and Sequence-indexed Lines of Arabidopsis Transposed from Start Loci on Chromosome 5
- AtIPT3 is a Key Determinant of Nitrate-Dependent Cytokinin Biosynthesis in Arabidopsis
- Expression and Interaction Analysis of Arabidopsis Skp1-Related Genes
- Hyperosmotic Stress Induces a Rapid and Transient Increase in Inositol 1,4,5-Trisphosphate Independent of Abscisic Acid in Arabidopsis Cell Culture
- ANALYSES OF PHOSPHOLIPASE C THAT FUNCTION IN HYPEROSMOTIC STRESS SIGNAL TRANSDUCTION CASCADE IN ARABIDOPSIS CELL CULTURE
- An Arabidopsis Gene Encoding a Ca^-Binding Protein is Induced by Abscisic Acid during Dehydration
- ANALYSES OF TRANSIENT ACTIVATION OF PHSPHOLIPASE C IN RESPONSE TO OSMOTIC STRESS IN ARABIDOPSIS CULTURED CELL
- Plastidic RNA polymerase σ factors in Arabidopsis
- Molecular Responses to Water Stress in Arabidopsis thaliana
- Analysis of ABA-dependent regulation of drought-responsive gene expression by bZIP transcription factor AREB in Arabidopsis
- MONITORING EXPRESSION PROFILES OF OSMOTIC STRESS-INDUCIBLE GENES USING cDNA MIRCROARRAY IN RICE
- rd22BP1 (MYC) and ATMYB2 (MYB) Act as Transcriptional Activators in ABA Signaling
- Analysis of bZIP transcriptional factor AREB involved in drought-responsive, ABA-dependent gene expression in Arabidopsis.
- MONITORING GENE EXPRESSION PATTERN UNDER DEHYDRATION AND COLD STRESS USING ARABIDOPSIS FULL-LENGTH cDNA MICROARRAY
- ANALYSIS OF AREB GENES WHICH INVOLVED IN THE DEHYDRATION AND ABA-RESPONSIVE EXPRESSION OF THE RD29B GENE IN ARABIDOSIS.
- Analysis of the Regulation of Drough-inducible Gene Expression of Arabidopsis rd22 Gene.
- BINDING SPECIFICITY OF DRE BINDING PROTEINS, DREBs, INVOLVED IN DEHYDRATION-RESPONSIVE TRANSCRIPTION IN ARABIDPOSIS.
- CHARACTERIZATION OF GENES ENCODING DEHYDRATION-RESPONSIVE-ELEMENT (DRE) BINDING PROTEINS
- Functional Analysis of an Activation Domein of an Arabidopsis MYC Homologue rd22BP1
- MOLECULAR CLONING OF ARABIDPSIS cDNAS ENCODING DNA BINDING PROTEINS THAT SPECIFICALLY BIND TO DEHYDRATION-RESPONSIVE ELEMENT (DRE)
- ANALYSES OF THE rd22BP1 GENE ENCODING MYC-RELATED PROTEIN THAT SPECIFICALY BINDS TO A CIS-ACTING ELEMENT INVOLVED IN DEHYDRATION-RESPONSIVE EXPRESSION OF THE rd22 GENE IN ARABIDOPSIS
- Isolation and Characterization of Novel Mutants Affecting the Abscisic Acid Sensitivity of Arabidopsis Germination and Seedling Growth
- A Novel Ethanol-Hypersensitive Mutant of Arabidopsis
- A Novel Arabidopsis Gene Required for Ethanol Tolerance is Conserved Among Plants and Archaea
- DNA Microarray Analysis of Plastid Gene Expression in an Arabidopsis Mutant Deficient in a Plastid Transcription Factor Sigma, SIG2
- ANALYSIS OF COMPONENTS OF THE PLASTID PROTEIN IMPORT APPARATUS IN ARABIDOPSIS
- Function and localization of Fab1 in Arabidopsis thaliana
- Functions of Endoxy loglucan Transferase, EXGT-A2 and EXGT-A3 in Arabidopsis thaliana
- Doping effect of Dy on leakage current and oxygen sensing property of SrTiO3 thin film prepared by PLD
- MOLECULAR CLONING OF AN ARABIDOPSIS HYBRID-TYPE HISTIDINE KINASE ATHK1
- A Combination of the Arabidopsis DREB1A Gene and Stress-Inducible rd29A Promoter Improved Drought- and Low-Temperature Stress Tolerance in Tobacco by Gene Transfer
- DREB genes in Rice, Oryza sativa L.
- Promoter analysis of ProDH gene induced by hypoosmolarity and L-Pro in Arabidopsis thaliana
- Functional analysis of Arabidopsis Response Regulators, ATRR1/ARR4/IBC7 and ATRR3/ARR8 in transgenic plants
- ANALYSIS OF TRANSGENIC PLANTS OVEREXPRESSING DOMINANT-NEGATIVE ATHK1 cDNAs IN ARABIDOPSIS
- ISOLATION AND CHARACTERIZATION OF Arabidopsis MUTANTS ALTERED IN COLD-, SALINITY-, AND ABA-INDUCTION OF rd29A GENE EXPRESSION
- The DREB family of genes in Rice, Oryza sativa
- REGULATION OF PROLINE BIOSYNTHESIS AND DEGRADATION IN TRANSGENIC ARABIDOPSIS
- Analysis of overexpression of DREB1A and DREB2A cDNAs for transcription factors involved in dehydration and cold responsive gene expression in transgenic Arabidopsis
- CHARACTERIZATION OF cDNA CLONES THATCORRESPOND TO GENES THAT RESPOND RAPIDLY TO DEHYDRATION INARABIDOPSIS THALIANA (III)
- Activation Energy of Oxygen Vacancy Diffusion of Yttria-Stabilized-Zirconia Thin Film Determined from DC Current Measurements below 150℃
- Orientation Control and Properties of Pb(Zr, Ti)O_3 Thin Films Deposited on Ni-Zn-Ferrite for Novel Ferroelectric/Ferromagnetic Memory Applications
- Effect of Yttria-Stabilized Zirconia Thickness on Crystal Structure and Electric Property of Epitaxial CeO_2/Yttria-Stabilized Buffer Layer in Metal/Ferroelectric/Insulator/Semiconductor Structure
- Preparation of PbTiO_3 Thin Film by Mist Source Plasma Enhanced Chemical Vapor Deposition (CVD) Using Heptane Solvent
- Identification of clp Genes Expressed in Senescing Arabidopsis Leaves
- Role of the Putative Membrane-Bound Endo-1,4-β-Glucanase KORRIGAN in Cell Elongation and Cellulose Synthesis in Arabidopsis thaliana
- A MEMBRANE-BOUND ENDO-1,4-β-GLUCANASE IS REQUIRED FOR CELLULOSE BIOSYNTHESIS IN ARABIDOPSIS
- CELL WALL ANALYSIS OF TEMPERATURE SENSITIVE MUTANT acwl OF A. thaliana
- Functional Analysis of Rice DREB1/CBF-type Transcription Factors Involved in Cold-responsive Gene Expression in Transgenic Rice
- Construction of a full-length cDNA library from young spikelets of hexaploid wheat and its characterization by large-scale sequencing of expressed sequence tags
- Mapping of Ds-transposon insertion sites transposed from start lines on Arabidopsis chromosome 5
- Functional analysis of galactinol synthase in A rabidopsis in drought stress tolerance
- FULL-LENGTH cDNA ACTIVATION SYSTEM OF PHYSCOMITRELLA PATENS TO STUDY AUXIN AND CYTOKININ FUNCTION
- Regional Insertional Mutagenesis of Specific Genes on the CIC5F11/CIC2B9 Locus of Arabidopsis thaliana Chromosome 5 Using the Ac/Ds Transposon in Combination with the cDNA Scanning Method
- The cDNA Microarray Analysis Using an Arabidopsis pad3 Mutant Reveals the Expression Profiles and Classification of Genes Induced by Alternaria brassicicola Attack
- A Ti-plasmid-convertible λ Phage Vector System Harboring the Hygromycin B Phosphotransferase Gene
- Cloning and Functional Analysis of a Novel DREB1/CBF Transcription Factor Involved in Cold-Responsive Gene Expression in Zea mays L
- ALBINO AND PALE GREEN 10 Encodes BBMII Isomerase Involved in Histidine Biosynthesis in Arabidopsis thaliana
- ABA-Activated SnRK2 Protein Kinase is Required for Dehydration Stress Signaling Arabidopsis
- Oxidative Stress Activates ATMPK6, an Arabidopsis Homologue of MAP Kinase
- MOLECULAR ANALYSES OF ARABIDOPSIS TWO-COMPONENT SYSTEMS
- FUNCTIONAL ANALYSIS OF ARABIDOPSIS ATHP1-3 GENES THAT ENCODE PHOSPHORELAY MEDIATORS
- Mapping of 25 Drought-Inducible Genes, RD and ERD, in Arabidopsis thaliana
- Early Salt-Stress Effects on Expression of Genes for Aquaporin Homologues in the Halophyte Sea Aster(Aster tripolium L. )
- MOLECULAR CLONING OF NOVEL ARABIDOPSIS GENES FOR TWO-COMPONENT SYSTEM PROTEINS THAT CONTAINS HPT DOMAIN
- Characterization of Two cDNAs for Novel Drought-Inducible Genes in the Highly Drought-Tolerant Cowpea
- RD28-LIKE GENES INDUCED BY SALT STRESS IN THE HALOPHYTE SEA ASTER
- Analysis of Phosphorylation of Wheat Elongation Factor 1β and β' by Casein Kinase II
- ANALYSIS OF DOMINANT-NEGATIVE ATHK1 IN ARABIDOPSIS
- Regulation of Levels of Proline as an Osmolyte in Plants under Water Stress
- Novel Drought-Inducible Genes in the Highly Drought-Tolerant Cowpea: Cloning of cDNAs and Analysis of the Expression of the Corresponding Genes
- Cloning of a Carrot cDNA for a Member of the Family of ADP-ribosylation Factors(ARFs) and Characterization of the Binding of Nucleotides by its Product after Expression in E. coli : GROWTH AND DEVELOPMENT : GENE STRUCTURE AND EXPRESSION
- The MALE STERILITY1 Gene of Arabidopsis, Encoding a Nuclear Protein with a PHD-finger Motif, is Expressed in Tapetal Cells and is Required for Pollen Maturation
- Immunoproteomic and Two-Dimensional Difference Gel Electrophoresis Analysis of Arabidopsis Dehydration Response Element-Binding Protein 1A (DREB1A)-Transgenic Potato
- ANALYSIS OF cDNAs FOR GENES THAT ARERESPONSIVE DROUGHT STRESS IN COWPEA
- Characterization of Two cDNAs(ERD10 and ERD14)Corresponding to Genes That Respond Rapidly to Dehydration Stress in Arabidopsis thaliana : ENVIRONMENTAL AND STRESS RESPONSES : GENES STRUCTURE AND EXPRESSION
- Three SnRK2 Protein Kinases are the Main Positive Regulators of Abscisic Acid Signaling in Response to Water Stress in Arabidopsis
- Chlorophyll b-Deficient Mutants of Rice : II. Antenna Chlorophyll a/b-Proteins of Photosystem I and II
- Chlorophyll b-Deficient Mutants of Rice : I. Absorption and Fluorescence Spectra and Chlorophyll a/b Ratios
- Three Arabidopsis SnRK2 Protein Kinases, SRK2D/SnRK2.2, SRK2E/SnRK2.6/OST1 and SRK2I/SnRK2.3, Involved in ABA Signaling are Essential for the Control of Seed Development and Dormancy
- Two Closely Related Subclass II SnRK2 Protein Kinases Cooperatively Regulate Drought-Inducible Gene Expression
- Stoichiometries of Photosystem I and Photosystem II in Rice Mutants Differently Deficient in Chlorophyll b
- BALANCE IN LIGHT ABSORPTION AND ELECTRON TRANSPORT BETWEEN PSI AND PS II IN CHLOROPHYLL b-DEFICIENT MUTANTS OF RICE
- Molecular Basis of the Core Regulatory Network in ABA Responses : Sensing, Signaling and Transport
- Antenna Sizes of Photosystem I and Photosystem II in Chlorophyll b-Deficient Mutants of Rice. Evidence for an Antenna Function of Photosystem II Centers That are Inactive in Electron Transport