Utilization of a floral organ-expressing AP1 promoter for generation of new floral traits in Torenia fournieri Lind
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概要
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To establish an efficient way to create novel floral traits in horticultural flowers, we have introduced many chimeric repressors of Arabidopsis transcription factors into torenia. Among them, we found a transgenic torenia exhibiting unopened flower buds and glossy dark green leaves with curled margins as a consequence of overexpression of Arabidopsis MYB24 with a transcriptional repression domain (MYB24-SRDX). Petals inside the flower buds exhibited a distinct coloration pattern. To bring out this favorable petal trait without inducing the unfavorable phenotypes due to the constitutive expression of chimeric repressors by the cauliflower mosaic virus 35S (35S) promoter, we tested the ability of a floral organ-specific Arabidopsis APETALA1 (AP1) promoter, which was found to be active in both petals and flower buds of torenia. As expected, AP1 pro:MYB24-SRDX transgenic torenias resulted in the opening of flowers and a normal leaf phenotype. Furthermore, these AP1 pro:MYB24-SRDX torenias exhibited wavy petals with a characteristic configuration. This is a good example of the utilization of a floral organ-specific promoter for creating distinct flower phenotypes without causing unfavorable morphological and physiological changes in other organs.
- 日本植物細胞分子生物学会の論文
著者
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OHTSUBO Norihiro
National Institute of Crop Science
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SHINOZAKI Kazuo
Inst Physic Chem Res (RIKEN)
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Shinozaki Kazuo
Laboratory Of Plant Molecular Biology Tsukuba Life Center Riken (the Institute Of Physical And Chemi
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Yamaguchi Hiroyasu
National Institute Of Floricultural Science
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SASAKI Katsutomo
National Institute of Floricultural Science
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Nakata Masaru
Bioproduction Res. Inst. National Inst. Of Advanced Industrial Sci. And Technol. (aist)
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Sage‐ono Kimiyo
Gene Res. Center Graduate School Of Life And Environmental Sciences Univ. Of Tsukuba
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Nakatsuka Takashi
Iwate Biotechnology Res. Center
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Narumi Takako
Faculty of Agriculture, Kagawa University
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Oshima Yoshimi
Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (
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Ohme-Takagi Masaru
Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (
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Mitsuda Nobutaka
Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (
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Shikat Masahito
National Institute of Floricultural Science, National Agriculture and Food Research Organization (NA
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Yamaguchi Hiroyasu
National Inst. Of Floricultural Sci. National Agriculture And Food Res. Organization (naro)
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Ohtsubo Norihiro
National Inst. Of Floricultural Sci. National Agriculture And Food Res. Organization (naro)
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Oshima Yoshimi
Bioproduction Res. Inst. National Inst. Of Advanced Industrial Sci. And Technol. (aist)
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Higuchi Youhei
National Inst. Of Floricultural Sci. National Agriculture And Food Res. Organization (naro)
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