High-efficiency improvement of transgenic torenia flowers by ion beam irradiation

スポンサーリンク

概要

• 論文の詳細を見る

• To shorten the time required for breeding and optimize the risk-cost/benefit ratio of genetically modified ornamental plants, we applied heavy-ion beam irradiation to wild-type and genetically modified torenia (Torenia fournieri Lind. CV. ‘Crown Violet’ plants in which petal color and pattern had been modified by controlling two anthocyanin biosynthesis-related genes encoding chalcone synthase (CHS) and dihydroflavonol-4-reductase (DFR). Ion beams of 12C6+ and 20Ne10+ were applied to 11,500 leaf disks from wild type and five transgenic lines, and over 3,200 regenerated flowering plants were then investigated for visible phenotypes. The mutation rate after whole irradiation averaged 10.4%, and the maximum rate in the initial screening was 44.2% (20Ne, 30 Gy). Mutant phenotypes were observed mainly in flowers and showed wide variation in color and shape. Mutation efficiencies for petal color and coloration pattern were higher in transgenic plants than in wild-type plants, while those for petal shape and corolla divergence were almost equivalent in the two plant groups. Mutation spectrums in petal color in transformant-based mutants were obviously wider than those in wild-type plants. Among these mutants, a class B gene-deficient mutant was investigated as a model case for further study to facilitate the control of flower phenotype. Expression of the TfGLO gene was found to be repressed in this line, probably due to dysfunctioning of the upstream signaling. We propose that the combination of genetic engineering and ion beam irradiation greatly facilitates improvement of agrobiological and commercial traits within a short period. We also discuss characteristic changes observed at high frequency in torenia flowers and the mutant-based approach to the identification of useful genes.

• 日本植物細胞分子生物学会の論文
• 2008-03-01

著者

• Shinozaki K

  Riken Ibaraki Jpn

• Shinozaki K

  Inst. Physical And Chemical Res. (riken) Ibaraki Jpn

• RYUTO Hiromichi

  Beam Technology Team, Nishina Accelerator Research Center, RIKEN

• FUKUNISHI Nobuhisa

  Beam Technology Team, Nishina Accelerator Research Center, RIKEN

• ABE Tomoko

  Radiation Biology Team, Nishina Accelerator Research Center, RIKEN

• Niki Tomoya

  National Institute of Agrobiological Resources

• Abe Tomoko

  Radiation Biology Team Nishina Accelerator Research Center Riken

• Abe Tomoko

  Riken Saitama Jpn

• Aida Ryutaro

  National Institute Of Floricultural Science National Agriculture And Food Research Organization

• Aida Ryutaro

  National Institute Of Floricultural Science National Agricultural Research Organization

• HAYASHI Yoriko

  RIKEN Nishina Center

• RYUTO Hiromichi

  RIKEN Nishina Center

• FUKUNISHI Nobuhisa

  RIKEN Nishina Center

• OHTSUBO Norihiro

  National Institute of Crop Science

• NISHIJIMA Takaaki

  National Research Institute of Vegetables, Ornamental Plants, and Tea, Ministry of Agriculture, Fore

• Shinozaki Kazuo

  Tsukuba Institute (RIKEN)

• HIGUCHI Yohei

  Graduate School of Life and Environmental Sciences, University of Tsukuba

• Ryuto Hiromichi

  Accelerator Research Program Frontier Research System Riken Institute

• Fukunishi Nobuhisa

  Beam Technology Team Nishina Accelerator Research Center Riken

• Yamaguchi Hiroyasu

  National Institute Of Floricultural Science

• Ohtsubo Norihiro

  National Institute Of Floricultural Science National Agriculture And Food Research Organization

• SASAKI Katsutomo

  National Institute of Floricultural Science

• NARUMI Takako

  National Institute of Floricultural Science

• Higuchi Youhei

  Univ. Tsukuba Ibaraki Jpn

• Abe Tomoko

  Riken

• Niki Tomoya

  National Institute Of Floricultural Science

• Saitoh Kumi

  Yamagata General Agricultural Research Center Department Of Agro-production Science

• Hayashi Yoriko

  Riken Saitama Jpn

• Higuchi Youhei

  Gene Research Center Graduate School Of Life And Environmental Sciences University Of Tsukuba

• TSUKATANI Nobue

  Iwate Biotechnology Research Center

• Yamaguchi Shinozaki

  Laboratory Of Plant Molecular Physiology Graduate School Of Agricultural And Life Sciences Universit

• Shinozaki Kazuo

  Lab. Of Pmb Riken

• Sage‐ono K

  Graduate School Of Life And Environmental Sciences Univ. Of Tsukuba

• Sage‐ono Kimiyo

  Gene Res. Center Graduate School Of Life And Environmental Sciences Univ. Of Tsukuba

• Nakatsuka Takashi

  Iwate Biotechnology Res. Center

• Yamaguchi Hiroyasu

  National Inst. Of Floricultural Sci. National Agriculture And Food Res. Organization (naro)

• Hayashi Yoriko

  Gene Research Center Graduate School Of Life And Environmental Sciences University Of Tsukuba

• Ohtsubo Norihiro

  National Inst. Of Floricultural Sci. National Agriculture And Food Res. Organization (naro)

• Nishijima Takaaki

  National Research Institute Of Vegetables Ornamental Plants And Tea

• Sage-ono Kimiyo

  Gene Res. Center Graduate School Of Life And Environmental Sciences Univ. Of Tsukuba

• Higuchi Youhei

  National Inst. Of Floricultural Sci. National Agriculture And Food Res. Organization (naro)

• Aida Ryutaro

  National Institute Of Floricultural Science

• Ohtsubo Norihiro

  National Inst. Of Floricultural Sci. (nifs) National Agriculture And Food Res. Organization (naro)

• Nishijima Takaaki

  National Institute Of Floricultural Science National Agriculture And Food Research Organization

関連論文

• Preparation of hydroxyapatite–ferrite composite particles by ultrasonic spray pyrolysis
• Characterization of Full-length Enriched Expressed Sequence Tags of Stress-treated Poplar leaves
• RELATION BETWEEN THE LEVEL OF SALT TOLERANCE AND THE LEVEL OF Na^+ ABSORPTION IN PROLINE-PRETREATED RICE (Oryza sativa L.) UNDER SALT STRESS
• Molecular Cloning and Characterization of a cDNA Encoding Proline Transporter in Rice
• Increase by water stress treatment and tissue specific localization of polyamines in Arabidopsis thaliana
• Expression Profiles of Arabidopsis Phospholipase A IIA Gene in Response to Biotic and Abiotic Stresses
• Profiling gene expression patterns under drought, cold and salt stress using Arabidopsis full-length cDNA microarray
• MOLECULAR ANALYSIS OF NOVEL DROUGHT-AND COLD-STRESS-INDUCIBLE GENES ISOLATED BY FULL-LENGTH-cDNA MICRO ARRAY SYSTEM IN ARABIDOPSIS
• A Resource of 5,814 Dissociation Transposon-tagged and Sequence-indexed Lines of Arabidopsis Transposed from Start Loci on Chromosome 5
• AtIPT3 is a Key Determinant of Nitrate-Dependent Cytokinin Biosynthesis in Arabidopsis
• Expression and Interaction Analysis of Arabidopsis Skp1-Related Genes
• Hyperosmotic Stress Induces a Rapid and Transient Increase in Inositol 1,4,5-Trisphosphate Independent of Abscisic Acid in Arabidopsis Cell Culture
• ANALYSES OF PHOSPHOLIPASE C THAT FUNCTION IN HYPEROSMOTIC STRESS SIGNAL TRANSDUCTION CASCADE IN ARABIDOPSIS CELL CULTURE
• An Arabidopsis Gene Encoding a Ca^-Binding Protein is Induced by Abscisic Acid during Dehydration
• ANALYSES OF TRANSIENT ACTIVATION OF PHSPHOLIPASE C IN RESPONSE TO OSMOTIC STRESS IN ARABIDOPSIS CULTURED CELL
• Plastidic RNA polymerase σ factors in Arabidopsis
• Analysis of ABA-dependent regulation of drought-responsive gene expression by bZIP transcription factor AREB in Arabidopsis
• MONITORING EXPRESSION PROFILES OF OSMOTIC STRESS-INDUCIBLE GENES USING cDNA MIRCROARRAY IN RICE
• rd22BP1 (MYC) and ATMYB2 (MYB) Act as Transcriptional Activators in ABA Signaling
• Analysis of bZIP transcriptional factor AREB involved in drought-responsive, ABA-dependent gene expression in Arabidopsis.
• MONITORING GENE EXPRESSION PATTERN UNDER DEHYDRATION AND COLD STRESS USING ARABIDOPSIS FULL-LENGTH cDNA MICROARRAY
• Isolation and Characterization of Novel Mutants Affecting the Abscisic Acid Sensitivity of Arabidopsis Germination and Seedling Growth
• A Novel Ethanol-Hypersensitive Mutant of Arabidopsis
• A Novel Arabidopsis Gene Required for Ethanol Tolerance is Conserved Among Plants and Archaea
• DNA Microarray Analysis of Plastid Gene Expression in an Arabidopsis Mutant Deficient in a Plastid Transcription Factor Sigma, SIG2
• ANALYSIS OF COMPONENTS OF THE PLASTID PROTEIN IMPORT APPARATUS IN ARABIDOPSIS
• Function and localization of Fab1 in Arabidopsis thaliana
• Functions of Endoxy loglucan Transferase, EXGT-A2 and EXGT-A3 in Arabidopsis thaliana
• Doping effect of Dy on leakage current and oxygen sensing property of SrTiO3 thin film prepared by PLD
• A Combination of the Arabidopsis DREB1A Gene and Stress-Inducible rd29A Promoter Improved Drought- and Low-Temperature Stress Tolerance in Tobacco by Gene Transfer
• DREB genes in Rice, Oryza sativa L.
• Promoter analysis of ProDH gene induced by hypoosmolarity and L-Pro in Arabidopsis thaliana
• Functional analysis of Arabidopsis Response Regulators, ATRR1/ARR4/IBC7 and ATRR3/ARR8 in transgenic plants
• ANALYSIS OF TRANSGENIC PLANTS OVEREXPRESSING DOMINANT-NEGATIVE ATHK1 cDNAs IN ARABIDOPSIS
• ISOLATION AND CHARACTERIZATION OF Arabidopsis MUTANTS ALTERED IN COLD-, SALINITY-, AND ABA-INDUCTION OF rd29A GENE EXPRESSION
• The DREB family of genes in Rice, Oryza sativa
• Activation Energy of Oxygen Vacancy Diffusion of Yttria-Stabilized-Zirconia Thin Film Determined from DC Current Measurements below 150℃
• Orientation Control and Properties of Pb(Zr, Ti)O_3 Thin Films Deposited on Ni-Zn-Ferrite for Novel Ferroelectric/Ferromagnetic Memory Applications
• Effect of Yttria-Stabilized Zirconia Thickness on Crystal Structure and Electric Property of Epitaxial CeO_2/Yttria-Stabilized Buffer Layer in Metal/Ferroelectric/Insulator/Semiconductor Structure
• Preparation of PbTiO_3 Thin Film by Mist Source Plasma Enhanced Chemical Vapor Deposition (CVD) Using Heptane Solvent
• Role of the Putative Membrane-Bound Endo-1,4-β-Glucanase KORRIGAN in Cell Elongation and Cellulose Synthesis in Arabidopsis thaliana
• A MEMBRANE-BOUND ENDO-1,4-β-GLUCANASE IS REQUIRED FOR CELLULOSE BIOSYNTHESIS IN ARABIDOPSIS
• Functional Analysis of Rice DREB1/CBF-type Transcription Factors Involved in Cold-responsive Gene Expression in Transgenic Rice
• Construction of a full-length cDNA library from young spikelets of hexaploid wheat and its characterization by large-scale sequencing of expressed sequence tags
• Mapping of Ds-transposon insertion sites transposed from start lines on Arabidopsis chromosome 5
• Functional analysis of galactinol synthase in A rabidopsis in drought stress tolerance
• FULL-LENGTH cDNA ACTIVATION SYSTEM OF PHYSCOMITRELLA PATENS TO STUDY AUXIN AND CYTOKININ FUNCTION
• Accumulation of Proline in the transgenic Arabidopsis overexpressing the DREB1A gene
• Cloning and Functional Analysis of a Novel DREB1/CBF Transcription Factor Involved in Cold-Responsive Gene Expression in Zea mays L
• Phenome analysis of root development in Arabidopsis
• Development of a novel method for characterization of cereals by spectral phenotyping
• ALBINO AND PALE GREEN 10 Encodes BBMII Isomerase Involved in Histidine Biosynthesis in Arabidopsis thaliana
• ABA-Activated SnRK2 Protein Kinase is Required for Dehydration Stress Signaling Arabidopsis
• Oxidative Stress Activates ATMPK6, an Arabidopsis Homologue of MAP Kinase
• Promoter analysis of erd1 : An Arabidopsis ClpAhomologous-gene, up-regulated in response to dark-induced senescence and dehydration stress
• Isolation of Arabidopsis DREB homologues that are involved in dehydration, cold and salt response, and functional analysis of DREB2A using Arabidopsis T-DNA insertion mutant
• CONSTRUCTION OF A FULL-LENGTH cDNA LIBRARY OF Arabidopsis thaliana USING BIOTINYLATED CAP TRAPPER
• High-efficiency improvement of transgenic torenia flowers by ion beam irradiation
• FioreDB : a database of phenotypic information induced by the chimeric repressor silencing technology (CRES-T) in Arabidopsis and floricultural plants
• A lead compound for the development of ABA 8″-hydroxylase inhibitors
• Differences between the structural requirements for ABA 8″-hydroxylase inhibition and for ABA activity
• Cytological and Biochemical Analysis of COF1, an Arabidopsis Mutant of an ABC Transporter Gene
• A Plant Growth Retardant, Uniconazole, Is a Potent Inhibitor of ABA Catabolism in Arabidopsis
• DEAR1, a transcriptional repressor of DREB protein that mediates plant defense and freezing stress responses in Arabidopsis
• Toward genome-wide metabolotyping and elucidation of metabolic system : metabolic profiling of large-scale bioresources
• Application of a metabolomic method combining one-dimensional and two-dimensional gas chromatography-time-of-flight/mass spectrometry to metabolic phenotyping of natural variants in rice
• Transcript Profiling of an Arabidopsis PSEUDO RESPONSE REGULATOR Arrhythmic Triple Mutant Reveals a Role for the Circadian Clock in Cold Stress Response
• Transcriptome Analyses Revealed Diverse Expression Changes in ago1 and hyl1 Arabidopsis Mutants
• PosMed-plus : An Intelligent Search Engine that Inferentially Integrates Cross-Species Information Resources for Molecular Breeding of Plants
• Alterations of Lysine Modifications on the Histone H3 N-Tail under Drought Stress Conditions in Arabidopsis thaliana
• Arabidopsis Transcriptome Analysis under Drought, Cold, High-Salinity and ABA Treatment Conditions using a Tiling Array
• Three SnRK2 Protein Kinases are the Main Positive Regulators of Abscisic Acid Signaling in Response to Water Stress in Arabidopsis
• Omics-Based Approaches to Methionine Side Chain Elongation in Arabidopsis : Characterization of the Genes Encoding Methylthioalkylmalate Isomerase and Methylthioalkylmalate Dehydrogenase
• Auxin Biosynthesis Inhibitors, Identified by a Genomics-Based Approach, Provide Insights into Auxin Biosynthesis
• Arabidopsis Bile Acid:Sodium Symporter Family Protein 5 is Involved in Methionine-Derived Glucosinolate Biosynthesis
• Widely Targeted Metabolomics Based on Large-Scale MS/MS Data for Elucidating Metabolite Accumulation Patterns in Plants
• ABA Hypersensitive Germination2-1 Causes the Activation of Both Abscisic Acid and Salicylic Acid Responses in Arabidopsis
• Three Arabidopsis SnRK2 Protein Kinases, SRK2D/SnRK2.2, SRK2E/SnRK2.6/OST1 and SRK2I/SnRK2.3, Involved in ABA Signaling are Essential for the Control of Seed Development and Dormancy
• Phenome Analysis in Plant Species Using Loss-of-Function and Gain-of-Function Mutants
• The Glycerophosphoryl Diester Phosphodiesterase-Like Proteins SHV3 and its Homologs Play Important Roles in Cell Wall Organization
• Aging effect on oxygen-sensitive electrical resistance of SrTiO3 thin films
• Two Closely Related Subclass II SnRK2 Protein Kinases Cooperatively Regulate Drought-Inducible Gene Expression
• ABA-mediated transcriptional regulation in response to osmotic stress in plants (JPR symposium: Opening a new era of ABA research)
• Visualization of metabolite identifier information
• Brz220 Interacts with DWF4, a Cytochrome P450 Monooxygenase in Brassinosteroid Biosynthesis, and Exerts Biological Activity
• Arabidopsis Seedlings Over-Accumulated Indole-3-acetic Acid in Response to Aminooxyacetic Acid
• Efficient Yeast One-/Two-Hybrid Screening Using a Library Composed Only of Transcription Factors in Arabidopsis thaliana
• Molecular Basis of the Core Regulatory Network in ABA Responses : Sensing, Signaling and Transport
• The new FioreDB database provides comprehensive information on plant transcription factors and phenotypes induced by CRES-T in ornamental and model plants
• Efficient production of novel floral traits in torenia by collective transformation with chimeric repressors of Arabidopsis transcription factors
• Trehalose drastically extends the in vitro vegetative culture period and facilitates maintenance of Torenia fournieri plants
• Utilization of a floral organ-expressing AP1 promoter for generation of new floral traits in Torenia fournieri Lind
• Arabidopsis chimeric TCP3 repressor produces novel floral traits in Torenia fournieri and Chrysanthemum morifolium
• Origin and evolution of genes related to ABA metabolism and its signaling pathways
• ABA-mediated transcriptional regulation in response to osmotic stress in plants
• RiceFOX : A Database of Arabidopsis Mutant Lines Overexpressing Rice Full-Length cDNA that Contains a Wide Range of Trait Information to Facilitate Analysis of Gene Function
• Arabidopsis RPT2α Encoding the 26S Proteasome Subunit is Required for Various Aspects of Root Meristem Maintenance, and Regulates Gametogenesis Redundantly with its Homolog, RPT2b
• Gene coding for SigA-binding protein from Arabidopsis appears to be transcriptionally up-regulated by salicylic acid and NPR1-dependent mechanisms
• An ABRE Promoter Sequence is Involved in Osmotic Stress-Responsive Expression of the DREB2A Gene, Which Encodes a Transcription Factor Regulating Drought-Inducible Genes in Arabidopsis
• Sugar-inducible RPT2a, a subunit of 26S proteasome, participates in sugar response in Arabidopsis

もっと見る 閉じる

スポンサーリンク