Efficient production of novel floral traits in torenia by collective transformation with chimeric repressors of Arabidopsis transcription factors
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概要
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Molecular breeding with genetic modification enables the production of novel floral traits in floricultural plants that could not be obtained by traditional breeding. To facilitate novel flower production, we collectively introduced 2 sets of 42 and 50 chimeric repressors of Arabidopsis transcription factors into Agrobacterium and then used these to co-transform torenia (Torenia fournieri). We generated 750 transgenic torenias, and identification of the transgenes revealed that more than 80% of the transgenic torenias had a single transgene. A total of 264 plants showed phenotypic modification, and 91.2% displayed modified flower colors and/or shapes, such as altered color patterns, curled petal margins, and wavy petals. These results indicated that the collective transformation system can be applied to molecular breeding of flowers. Detailed analysis of the phenotypes revealed that PETAL LOSS could control blotch sizes and that modification of cell shape could change the texture of petals. We found that the chimeric repressors of functionally unknown transcription factors also induced novel floral traits, and therefore, the transgenic torenias provide an understanding of the functions of transcription factors that could not be revealed by previous studies in Arabidopsis.
- 2011-03-25
著者
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Shinozaki K
Riken Plant Sci. Center Yokohama Jpn
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Shinozaki K
Riken Ibaraki Jpn
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Shinozaki K
Inst. Physical And Chemical Res. (riken) Ibaraki Jpn
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Aida Ryutaro
National Institute Of Floricultural Science National Agricultural Research Organization
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Ohme Takagi
Research Institute Of Genome-based Biofactory National Institute Of Advanced Industrial Science And
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SHINOZAKI Kazuo
Plant Mol. Biol. RIKEN
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Soeno Kazuo
RIKEN Plant Science Center
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SHIZOZAKI Kazuo
Plant Mol. Biol. RIKEN
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SHINOZAKI Kazuo
Inst Physic Chem Res (RIKEN)
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Yamaguchi Shinozaki
Biological Resources Division Japan International Research Center For Agricultural Sciences (jircas)
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Shinozaki Kazuo
Tsukuba Institute (RIKEN)
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HIGUCHI Yohei
Graduate School of Life and Environmental Sciences, University of Tsukuba
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Shinozaki Kazuo
Plant Mol. Biol. Lab.
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Shinozaki Kazuo
Laboratory Of Plant Molecular Biology Tsukuba Life Center Riken (the Institute Of Physical And Chemi
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Shinozaki K
Univ. Tsukuba Ibaraki Jpn
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SASAKI Katsutomo
National Institute of Floricultural Science
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Higuchi Youhei
Univ. Tsukuba Ibaraki Jpn
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Saitoh Kumi
Yamagata General Agricultural Research Center Department Of Agro-production Science
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Hayashi Yoriko
Riken Saitama Jpn
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Higuchi Youhei
Gene Research Center Graduate School Of Life And Environmental Sciences University Of Tsukuba
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TSUKATANI Nobue
Iwate Biotechnology Research Center
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Yamaguchi Shinozaki
Laboratory Of Plant Molecular Physiology Graduate School Of Agricultural And Life Sciences Universit
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Shinozaki Kazuo
Lab. Of Pmb Riken
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Sako Kaori
Faculty Of Advanced Life Science Graduate School Of Life Science Hokkaido University
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Sage‐ono K
Graduate School Of Life And Environmental Sciences Univ. Of Tsukuba
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Ohme Takagi
Research Institute Of Genome-based Biofactory National Institute Of Advanced Industrial Science And
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Shikata Masahito
National Institute of Floricultural Science, National Agriculture and Food Research Organization (NA
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Narumi Takako
Faculty of Agriculture, Kagawa University
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Takiguchi Yuko
Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (
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Mitsuda Nobutaka
Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (
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Yamaguchi Hiroyasu
National Inst. Of Floricultural Sci. National Agriculture And Food Res. Organization (naro)
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Ohme Takagi
Bioproduction Research Institute National Institute Of Advanced Industrial Science And Technology (a
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Ohtsubo Norihiro
National Inst. Of Floricultural Sci. National Agriculture And Food Res. Organization (naro)
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Oshima Yoshimi
Bioproduction Res. Inst. National Inst. Of Advanced Industrial Sci. And Technol. (aist)
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Sage-ono Kimiyo
Gene Res. Center Graduate School Of Life And Environmental Sciences Univ. Of Tsukuba
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Shibata Michio
National Institute Of Floricultural Science National Agriculture And Food Research Organization
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Mitsuda Nobutaka
Bioproduction Research Institute National Institute Of Advanced Industrial Science And Technology
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Shinozaki Kazuo
Riken Plant Sci. Center Yokohama Jpn
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Oshima Yoshimi
Bioproduction Research Institute National Institute Of Advanced Industrial Science And Technology
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Aida Ryutaro
National Institute Of Floricultural Science
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Ohme Takagi
Bioproduction Research Institute National Institute Of Advanced Industrial Science And Technology
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Saito Kazuki
Metabolomics Research Group Riken Plant Science Center
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Ohtsubo Norihiro
National Inst. Of Floricultural Sci. (nifs) National Agriculture And Food Res. Organization (naro)
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Takiguchi Yuko
Bioproduction Research Institute National Institute Of Advanced Industrial Science And Technology
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