Agrobacterium tumefaciens-mediated Transformation of Delphinium spp
スポンサーリンク
概要
- 論文の詳細を見る
A successful transformation method was reported on Delphinium spp., using Agrobacterium tumefaciens. Elongated hypocotyl and cotyledon petiole segments were infected with Agrobacterium tumefaciens strain LBA4404, which harbored a binary vector plasmid, pIG121-Hm, which included the β-glucuronidase (GUS) gene (with an intron) as a reporter gene, and the neomycin phosphotransferase II gene and the hygromycin phosphotransferase gene as selection markers. Explants were cultured on Murashige and Skoog medium supplemented with 1.0mgl-1 thidiazuron, 1.0mgl-1 2, 4-dichlorophenoxyacetic acid, 300mgl-1 ticarcillin, and 5mgl-1 hygromycin or 100mgl-1 kanamycin (selection medium) for regeneration. Transformation was confirmed by histochemical assays of GUS activity in plant tissues, and by PCR analysis of the GUS gene. Through four experiments, six independent GUS-positive regenerants were obtained out of 1276 explants.
- 日本植物細胞分子生物学会の論文
- 2002-12-03
著者
-
Aida Ryutaro
National Institute Of Floricultural Science National Agriculture And Food Research Organization
-
Aida Ryutaro
National Institute Of Floricultural Science National Agricultural Research Organization
-
Shibata Michio
National Institute Of Floricultural Science:graduate School Of Life And Environmental Sciences Unive
-
Shibata Michio
National Institute Of Floricultural Science National Agricultural Research Organization
-
Hirose Yukio
Ehime Agricultural Experiment Station
-
Aida Ryutaro
National Institute Of Floricultural Science
-
Shibata Michio
National Institute of Floricultural Science
関連論文
- High Frequency of Polyploidization in Regenerated Plants of Kalanchoe blossfeldiana Cultivar 'Tetra Vulcan'
- Importance of genetic transformation in ornamental plant breeding
- High-efficiency improvement of transgenic torenia flowers by ion beam irradiation
- Improved translation efficiency in chrysanthemum and torenia with a translational enhancer derived from the tobacco alcohol dehydrogenase gene
- Chimeric AGAMOUS repressor induces serrated petal phenotype in Torenia fournieri similar to that induced by cytokinin application
- FioreDB : a database of phenotypic information induced by the chimeric repressor silencing technology (CRES-T) in Arabidopsis and floricultural plants
- Efficient production of novel floral traits in torenia by collective transformation with chimeric repressors of Arabidopsis transcription factors
- PCR-RFLP Analysis of Chloroplast DNA Variations in the atpI-atpH Spacer Region of the Genus Camellia
- Efficient Transgene Expression in Chrysanthemum, Dendranthema grandiflorum (Ramat.) Kitamura, by Using the Promoter of a Gene for Chrysanthemum Chlorophyll-a/b-binding Protein
- Identification of Cyanidin 3-O-(3", 6"-O-Dimalonyl-β-glucopyranoside) as a Flower Pigment of Chrysanthemum (Dendranthema grandflorum)