An Effective Method for Detection and Analysis of DNA Damage Induced by Heavy-Ion Beams
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概要
- 論文の詳細を見る
We have developed an efficient system to detect and analyze DNA mutations induced by heavy-ion beams in Arabiopsis thaliana. In this system, a stable transgenic Arabidopsis line that constitutively expresses a yellow fluorescent protein (YFP) by a single-copy gene at a genomic locus was constructed and irradiated with heavy-ion beams. The YFP gene is a target of mutagenesis, and its loss of function or expression can easily be detected by the disappearance of YFP signals in planta under microscopy. With this system, a 12C6+-induced mutant with single deletion and multiple base changes was isolated.
著者
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Abe Tomoko
Riken Saitama Jpn
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Kazama Yusuke
RIKEN Nishina Center
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Saito Hiroyuki
RIKEN Nishina Center
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Fujiwara Makoto
RIKEN Nishina Center
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MATSUYAMA Tomoki
Cellular Biochemistry Laboratory, RIKEN
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HAYASHI Yoriko
RIKEN Nishina Center
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RYUTO Hiromichi
RIKEN Nishina Center
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FUKUNISHI Nobuhisa
RIKEN Nishina Center
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Saito Hiroyuki
Radiation Biology Team Nishina Accelerator Research Center Riken
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