Agrobacterium-mediated genetic transformation of Cattleya with an Odontoglossum ringspot virus replicase gene sequence
スポンサーリンク
概要
- 論文の詳細を見る
Protocorm-like bodies (PLBs) of Cattleya orchid CM2450 cultured either under constant illumination with cool-white-fluorescent lamps or in the dark were cocultivated with Agrobacterium tumefaciens strain EHA105 carrying plasmid pSMAHdN627-ORSV harboring genes coding for Odontoglossum ringspot virus (ORSV) replicase and hygromycin phosphotransferase. PLBs were maintained in liquid New Dogashima (ND) medium and then added to a bacterial suspension culture (OD600≈0.6) yielding medium dilution ratio of 1 : 10 and incubated for either 30 minutes or 3 h. Hygromycin-resistant secondary PLBs were induced after 4 weeks of culture on 2.5 g l−1 gellan gum-solidified ND medium containing 1 mg l−1 naphthaleneacetic acid (NAA), 0.1 mg l−1 benzyladenine (BA), 10 mg l−1 hygromycin, 20 mg l−1 meropenem, 10 g l−1 sucrose in both light- and dark-cultured PLBs. The number of resistant PLBs generated using dark-cultured PLBs was higher than those cultured under constant illumination. Presence of acetosyringone (AS) in the pre-culture medium was also effective for the transformation. The highest frequency of transformation was obtained when dark-cultured PLBs were pre-cultured with 100 μM AS for 3 days and inoculated with Agrobacterium liquid culture for 3 h. Transformation of hygromycin-resistant plantlets regenerated from different sites of inoculated PLBs was confirmed by Southern blot hybridization. Transcription of ORSV replicase gene in transgenic lines was successfully confirmed by Northern blot hybridization.
- 2010-12-25
著者
-
Chin Dong
Graduate School Of Horticulture Chiba Univ.
-
Chin Dong
Lab. Of Plant Cell Technol. Graduate School Of Horticulture Chiba Univ.
-
NAKAMURA Ikuo
Laboratory of Plant Cell Technology, Graduate School of Horticulture, Chiba University
-
Nakamura Ikuo
Lab. Of Plant Cell Technol. Graduate School Of Horticulture Chiba Univ.
-
Nakamura Ikuo
Graduate School Of Horticulture Chiba Univ.
-
FUKAMI Masanobu
Chiba Prefectural Agriculture and Forestry Research Center
-
Ichikawa Hiroaki
Division Of Genome And Biodiversity Research National Institute Of Agrobiological Sciences
-
Mii Masahiro
Graduate School Of Horticulture Chiba University
-
Zhang Lin
Graduate School Of Horticulture Chiba University
-
Nakamura Ikuo
Graduate School Of Agriculture Hokkaido University
-
Mii Masahiro
Graduate School Of Horticulture Chiba Univ.
関連論文
- Aegilops section Sitopsis species contains the introgressive PolA1 gene with a closer relationship to that of Hordeum than Triticum-Aegilops species
- Molecular discrimination among taxa of Petunia axillaris complex and P. integrifolia complex based on PolA1 sequence analysis
- Transgenic spinach plants produced by Agrobacterium-mediated method based on the low temperature-dependent high plant regeneration ability of leaf explants
- Generation of phenotypically normal marker-free transgenic plants of Kalanchoe blossfeldiana through hairy root induction
- Thidiazuron-induced rapid shoot regeneration via embryo-like structure formation from shoot tip-derived callus culture of sugarcane
- Promoter analysis of OsAMT1;2 and OsAMT1;3 implies their distinct roles in nitrogen utilization in rice
- Thermo-Resistive Platinum Thin Film Hydrogen Gas Sensor Fabricated by MEMS Techniques
- Agrobacterium-mediated transformation of spinach (Spinacia oleracea) with Bacillus thuringiensis cry1Ac gene for resistance against two common vegetable pests
- Development of Asexual Propagation System via in Vitro Culture in Cardamine yezoensis Maxim. and its Application to Hydroponic Cultivation
- Gametosomatic hybridization between egg cell protoplast and mesophyll protoplast of Petunia hybrida
- Direct isolation of female germ units from ovules of Petunia hybrida by enzymatic treatment without releasing somatic protoplasts from ovular tissue
- Transgenic Phalaenopsis plants with resistance to Erwinia carotovora produced by introducing wasabi defensin gene using Agrobacterium method
- Activation tagging approach in a model legume, Lotus japonicus
- Evolutionary analysis of two plastid DNA sequences in cultivated and wild species of Oryza
- Assessment of Genetic Diversity among the Major Myanmar Banana Landraces
- Rapid and simple DNA extraction method from rice using a glass-fiber filter inserted pipette tip
- Structure and characterization of the gene encoding α subunit of soybean β-conglycinin
- Transgenic potatoes expressing wasabi defensin peptide confer partial resistance to gray mold (Botrytis cinerea)
- Effect of heavy ion-beam irradiation on plant growth and mutation induction in Nicotiana tabacum
- Production and characterization of a large population of cDNA-overexpressing transgenic rice plants using Gateway-based full-length cDNA expression libraries
- Rice transgenic resources with gain-of-function phenotypes
- Ectopic Overexpression of The Transcription Factor OsGLK1 Induces Chloroplast Development in Non-Green Rice Cells
- Unreduced gametes are the major causal factor for the production of polyploid interspecific hybrids in Primula
- Recent advances in wide hybridization and embryo rescue of floricultural plants in Japan
- Thidiazuron-induced rapid shoot regeneration via embryo-like structure formation from shoot tip-derived callus culture of sugarcane
- Agrobacterium-mediated genetic transformation of Cattleya with an Odontoglossum ringspot virus replicase gene sequence
- Overproduction of artemisinin in tetraploid Artemisia annua L.
- Interspecific hybridization of Begonia semperflorens (section Begonia) with B. pearcei (section Eupetalum) for introducing yellow flower color
- Ionic compositions play an important role on in vitro propagation of PLBs of spring-flowering Calanthe
- Genetic engineering of chrysanthemum (Chrysanthemum morifolium): Current progress and perspectives
- Plant regeneration from Crotalaria spectabilis hairy roots which showed inhibited growth of root-knot nematodes (Meloidogyne hapla and M. incognita) in vitro
- Transgenic Petunia hybrids expressing a synthetic fungal chitinase gene confers disease tolerance to Botrytis cinerea
- Genetic engineering of chrysanthemum (Chrysanthemum morifolium) : Current progress and perspectives
- Population structural analysis of an in-situ conservation site for wild rice in Laos
- Highly efficient system for plant regeneration from leaf and stem explants in Dahlia
- Establishment of Agrobacterium-mediated genetic transformation system in Dahlia
- Characterization of lignocellulose of Erianthus arundinaceus in relation to enzymatic saccharification efficiency
- Overexpression of a Rice TIFY Gene Increases Grain Size through Enhanced Accumulation of Carbohydrates in the Stem
- Efficient plant regeneration system from seed-derived callus of ravenna grass [Erianthus ravennae (L.) Beauv.]
- Disruption of GSTZ1 Gene by Large Genetic Alteration in Oryza glaberrima
- Population structural analysis of an in-situ conservation site for wild rice in Laos
- Characterization of lignocellulose of Erianthus arundinaceus in relation to enzymatic saccharification efficiency