Highly efficient system for plant regeneration from leaf and stem explants in Dahlia
スポンサーリンク
概要
- 論文の詳細を見る
Protocol of plant regeneration from leaf and stem explants was established in dahlia (Dahlia×pinnata) 'Yamatohime' in in vitro culture. Nodular calli were successfully induced from leaf and stem explants on 30 g l−1 sucrose-containing MS medium supplemented singly with one of the cytokinins, 6-benzyladenine (BA), zeatin or thidiazuron (TDZ) and the highest frequency of nodular callus induction was obtained on medium containing 10 mg l−1 TDZ. The nodular callus induced on this medium subsequently produced a tissue with numerous shoot primordial structures called 'mass of shoot primordia' (MSP), which was maintained at least for more than 2 years on the same medium. Shoot induction occurred after transfer the MSP onto hormone-free MS medium, in which replacement of sucrose by maltose was necessary. The shoots excised from the MSP were rooted on hormone-free MS medium and successfully grown under greenhouse conditions. The regenerated plants showed the same morphological characteristics to those of the control plants and flowered normally.
著者
-
Chin Dong
Graduate School Of Horticulture Chiba Univ.
-
Otani Yuko
Graduate School Of Pharmaceutical Sciences The University Of Tokyo
-
Mii Masahiro
Graduate School Of Horticulture Chiba Univ.
-
Endo Chuki
Graduate School of Horticulture, Chiba University
関連論文
- Transgenic spinach plants produced by Agrobacterium-mediated method based on the low temperature-dependent high plant regeneration ability of leaf explants
- Generation of phenotypically normal marker-free transgenic plants of Kalanchoe blossfeldiana through hairy root induction
- Thidiazuron-induced rapid shoot regeneration via embryo-like structure formation from shoot tip-derived callus culture of sugarcane
- Dependence of Estrogenic Activity on the Shape of the 4-Alkyl Substituent in Simple Phenols
- Agrobacterium-mediated transformation of spinach (Spinacia oleracea) with Bacillus thuringiensis cry1Ac gene for resistance against two common vegetable pests
- Gametosomatic hybridization between egg cell protoplast and mesophyll protoplast of Petunia hybrida
- Direct isolation of female germ units from ovules of Petunia hybrida by enzymatic treatment without releasing somatic protoplasts from ovular tissue
- Transgenic Phalaenopsis plants with resistance to Erwinia carotovora produced by introducing wasabi defensin gene using Agrobacterium method
- Transgenic potatoes expressing wasabi defensin peptide confer partial resistance to gray mold (Botrytis cinerea)
- Effect of heavy ion-beam irradiation on plant growth and mutation induction in Nicotiana tabacum
- Synthesis of Nonplanar Peptides Bearing the 7-Azabicyclo[2. 2. 1]heptane Skeleton, and the Possible Self-organized Structures
- Unreduced gametes are the major causal factor for the production of polyploid interspecific hybrids in Primula
- Recent advances in wide hybridization and embryo rescue of floricultural plants in Japan
- Thidiazuron-induced rapid shoot regeneration via embryo-like structure formation from shoot tip-derived callus culture of sugarcane
- Agrobacterium-mediated genetic transformation of Cattleya with an Odontoglossum ringspot virus replicase gene sequence
- Overproduction of artemisinin in tetraploid Artemisia annua L.
- Interspecific hybridization of Begonia semperflorens (section Begonia) with B. pearcei (section Eupetalum) for introducing yellow flower color
- Ionic compositions play an important role on in vitro propagation of PLBs of spring-flowering Calanthe
- Plant regeneration from Crotalaria spectabilis hairy roots which showed inhibited growth of root-knot nematodes (Meloidogyne hapla and M. incognita) in vitro
- Transgenic Petunia hybrids expressing a synthetic fungal chitinase gene confers disease tolerance to Botrytis cinerea
- Highly efficient system for plant regeneration from leaf and stem explants in Dahlia
- Establishment of Agrobacterium-mediated genetic transformation system in Dahlia
- Characterization of lignocellulose of Erianthus arundinaceus in relation to enzymatic saccharification efficiency
- Efficient plant regeneration system from seed-derived callus of ravenna grass [Erianthus ravennae (L.) Beauv.]
- Characterization of lignocellulose of Erianthus arundinaceus in relation to enzymatic saccharification efficiency