Efficient plant regeneration system from seed-derived callus of ravenna grass [Erianthus ravennae (L.) Beauv.]
スポンサーリンク
概要
- 論文の詳細を見る
An efficient method of plant regeneration from seed-derived callus was established in ravenna grass [Erianthus ravennae (L.) Beauv.], which is an ornamental tall grass as well as an important biomass crop. For callus induction, mature seeds were cultured on media containing different concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) (0.5, 1, 2 and 4 mg l−1) and 6-benzyladenine (BA) (0, 0.1 and 0.2 mg l−1) on callus induction using MS medium (Murashige and Skoog 1962) supplemeted with 1 g l−1 casamino acids, 30 g l−1 maltose and 8 g l−1 agar as a basal medium. The highest callus induction was obtained on medium supplemented with 2 mg l−1 2,4-D and 0.2 mg l−1 BA, where 96% of explants formed callus. During the subculture of the calli on the same medium for 4 months, 3 types of calli showing different growth rate, color and morphology were differentiated. By using these 3 types of calli, effects of different concentrations of BA or 6-furfurylaminopurine (kinetin) (0, 0.1, 0.5, 1, 2 and 4 mg l−1) and naphthaleneacetic acid (NAA) (0, 0.25, 0.5 and 1 mg l−1) as plant growth regulators on shoot regeneration were evaluated using MS medium with 1 g l−1 casamino acids as a basal medium. The highest frequency of shoot formation was obtained in type A callus, which had white and compact nature, on medium containing 30 g l−1 sucrose, 1 mg l−1 BA and 0.1–0.25 mg l−1 NAA, where 89.3% of the calli formed shoots.
- 日本植物細胞分子生物学会の論文
著者
-
Chin Dong
Graduate School Of Horticulture Chiba Univ.
-
Mii Masahiro
Graduate School Of Horticulture Chiba Univ.
-
Khan Raham
Graduate School of Horticulture, Chiba University
-
Shimomae Kazuki
Graduate School of Horticulture, Chiba University
関連論文
- Transgenic spinach plants produced by Agrobacterium-mediated method based on the low temperature-dependent high plant regeneration ability of leaf explants
- Generation of phenotypically normal marker-free transgenic plants of Kalanchoe blossfeldiana through hairy root induction
- Thidiazuron-induced rapid shoot regeneration via embryo-like structure formation from shoot tip-derived callus culture of sugarcane
- Agrobacterium-mediated transformation of spinach (Spinacia oleracea) with Bacillus thuringiensis cry1Ac gene for resistance against two common vegetable pests
- Gametosomatic hybridization between egg cell protoplast and mesophyll protoplast of Petunia hybrida
- Direct isolation of female germ units from ovules of Petunia hybrida by enzymatic treatment without releasing somatic protoplasts from ovular tissue
- Transgenic Phalaenopsis plants with resistance to Erwinia carotovora produced by introducing wasabi defensin gene using Agrobacterium method
- Transgenic potatoes expressing wasabi defensin peptide confer partial resistance to gray mold (Botrytis cinerea)
- Effect of heavy ion-beam irradiation on plant growth and mutation induction in Nicotiana tabacum
- Unreduced gametes are the major causal factor for the production of polyploid interspecific hybrids in Primula
- Recent advances in wide hybridization and embryo rescue of floricultural plants in Japan
- Thidiazuron-induced rapid shoot regeneration via embryo-like structure formation from shoot tip-derived callus culture of sugarcane
- Agrobacterium-mediated genetic transformation of Cattleya with an Odontoglossum ringspot virus replicase gene sequence
- Overproduction of artemisinin in tetraploid Artemisia annua L.
- Interspecific hybridization of Begonia semperflorens (section Begonia) with B. pearcei (section Eupetalum) for introducing yellow flower color
- Ionic compositions play an important role on in vitro propagation of PLBs of spring-flowering Calanthe
- Plant regeneration from Crotalaria spectabilis hairy roots which showed inhibited growth of root-knot nematodes (Meloidogyne hapla and M. incognita) in vitro
- Transgenic Petunia hybrids expressing a synthetic fungal chitinase gene confers disease tolerance to Botrytis cinerea
- Highly efficient system for plant regeneration from leaf and stem explants in Dahlia
- Establishment of Agrobacterium-mediated genetic transformation system in Dahlia
- Characterization of lignocellulose of Erianthus arundinaceus in relation to enzymatic saccharification efficiency
- Efficient plant regeneration system from seed-derived callus of ravenna grass [Erianthus ravennae (L.) Beauv.]
- Characterization of lignocellulose of Erianthus arundinaceus in relation to enzymatic saccharification efficiency