Molecular discrimination among taxa of Petunia axillaris complex and P. integrifolia complex based on PolA1 sequence analysis

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概要

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• Petunia axillaris complex and P. integrifolia complex consist of three subspecies (ssp. axillaris, ssp. parodii and ssp. subandina) and two species (P. integrifolia and P. inflata), respectively. Since these taxa within each complex can only be distinguished by the flower morphology, it would be useful to discriminate them by DNA markers. In this study, we sequenced the 19th intron and the 20th exon of the PolA1 gene, a single gene encoding the largest subunit of RNA polymerase I, and showed clear differences between P. axillaris complex and P. integrifolia complex, and also among all the taxa within each complex. These sequence variations will be promising to develop PCR-based markers to discriminate taxa within P. axillaris complex and P. integrifolia complex.

著者

• NAKAMURA Ikuo

  Laboratory of Plant Cell Technology, Graduate School of Horticulture, Chiba University

• Nakamura Ikuo

  Lab. Of Plant Cell Technol. Graduate School Of Horticulture Chiba Univ.

• Nakamura Ikuo

  Faculty Of Engineering Nagoya University

• MII Masahiro

  Faculty of Horticulture, Chiba University

• TAKAHASHI Hiroko

  Graduate School of Horticulture, Chiba University

• Takahashi Hiroko

  Graduate School Of Horticulture Chiba University

• Mii Masahiro

  Faculty Of Horticulture Chiba University

• Zhang Xinwang

  Graduate School of Science and Technology, Chiba University

• Zhang Xinwang

  Graduate School Of Science And Technology Chiba University

• Takahashi Hiroko

  Graduate School Of Environmental Studies Tohoku University

• Nakamura Ikuo

  Faculty Mechanical Engineering Nagoya University

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