Disruption of GSTZ1 Gene by Large Genetic Alteration in Oryza glaberrima
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概要
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After the completion of the genome sequencing project of common rice (Oryza sativa L.), comparative genomic studies between rice and related species became important to reveal the function of each gene. The rice genome contains two copies of the gene encoding zeta class glutathione S-transferase (GSTZ) that is reported to be the enzyme in the catabolic pathway of tyrosine and phenylalanine. Two GSTZ genes of O. sativa, OsGSTZ1 and OsGSTZ2, display a tandem arrangement. Up-stream OsGSTZ1 gene is constitutively expressed, whereas the downstream OsGSTZ2 gene is inducible by stresses. We analyzed the expression of the GSTZ gene in the African cultivated species O. glaberrima and wild species O. longistaminata by using RT-PCR. The results showed that both GSTZ1 and GSTZ2 genes were expressed in O. longistaminata, whereas only the cDNA fragment of downstream GSTZ2 was detected in O. glaberrima. Thus, the genomic sequence of the GSTZ1 locus of O. glaberrima was determined by PCR genomic walking. Sequence comparison between O. sativa and O. glaberrima revealed that the genomic sequence up-stream from the eighth intron of the OgGSTZ1 gene was highly homologous, in inverted orientation, to a BAG clone, over 1-Mb apart from the OsGSTZ1 gene in O. sativa. This result suggested that the OgGSTZ1 gene was disrupted by a large rearrangement or inversion. The genetic alteration was also observed in several lines of O. glaberrima and its ancestral wild species O. barthii. The loss of the OgGSTZ1 gene in O. glaberrima is important, as the expression of the gene is 12-fold higher than that of OsGSTZ2 in O. sativa and since O. sativa and O. glaberrima differ in several such as seed yield and annual/perennial habitat that might be related to the translocation of nitrogen metabolites from leaves to seeds.
- 日本育種学会の論文
- 2004-03-01
著者
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Nakamura Ikuo
Graduate School Of Agriculture Hokkaido University
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Tsuchiya Tokuji
Graduate School of Science and Technology, Chiba University
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- Disruption of GSTZ1 Gene by Large Genetic Alteration in Oryza glaberrima
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