Cloning and Sequencing of the ura3 and ura5 Genes, and Isolation and Characterization of Uracil Auxotrophs of the Fungus Mortierella alpina 1S-4
スポンサーリンク
概要
- 論文の詳細を見る
The oil-producing fungus Mortierella alpina 1S-4 is an industrial strain. In order to prepare host strains for a transformation system for this fungus, six uracil auxotrophs were obtained by means of random mutation with N-methyl-N′-nitro-N-nitrosoguanidine (MNNG). When the activities of orotate phosphoribosyl transferase (OPRTase, EC 2.4.2.10) and orotidine-5′-phosphate decarboxylase (OMPdecase, EC 4.1.1.23) were examined in the mutants and wild strain, OPRTase activity was found to be completely absent in all mutants, on the other hand, OMPdecase activity was intact. The genomic DNA and cDNA of the ura5 gene encoding OPRTase and the ura3 gene encoding OMPdecase were cloned and sequenced. The Ura5p deduced amino acid sequence of this fungus showed highest similarity to that of Vibrio cholerae classed among prokaryote. Furthermore, the mutational points in the ura5 genes of two selected mutants were identified; a base-replacement and a base-insertion.
- 社団法人 日本農芸化学会の論文
- 2004-02-23
著者
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Sakuradani Eiji
Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University
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Sakuradani E
Kyoto Univ. Kyoto Jpn
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SAKURADANI Eiji
Laboratory of Fermentation Physiology and Applied Microbiology, Division of Applied Life Sciences, G
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SHIMIZU Sakayu
Laboratory of Fermentation Physiology and Applied Microbiology, Division of Applied Life Sciences, G
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Takeno Seiki
Laboratory of Fermentation Physiology and Applied Microbiology, Division of Applied Life Sciences, G
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Kawashima Hiroshi
Institute for Health Care Science, Suntory Ltd.
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MURATA Shoichi
Laboratory of Fermentation Physiology and Applied Microbiology, Division of Applied Life Sciences, G
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INOHARA (OCHIAI)
Institute for Advanced Technology, Suntory Ltd.
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ASHIKARI Toshihiko
Institute for Advanced Technology, Suntory Ltd.
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Sakuradani Eiji
Laboratory Of Fermentation Physiology And Applied Microbiology Division Of Applied Life Sciences Gra
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Shimizu Sakayu
Laboratory Of Fermentation Physiology And Applied Microbiology Division Of Applied Life Sciences Gra
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Takeno Seiki
Laboratory Of Fermentation Physiology And Applied Microbiology Division Of Applied Life Sciences Gra
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Murata Shoichi
Laboratory Of Fermentation Physiology And Applied Microbiology Division Of Applied Life Sciences Gra
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Ashikari Toshihiko
Institute For Advanced Technology Suntory Ltd.
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Inohara (ochiai)
Institute For Advanced Technology Suntory Ltd.
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Kawashima Hiroshi
Institute For Health Care Science Suntory Ltd.
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Kawashima Hiroshi
Institute For Fundamental Research Suntory Ltd.
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