Identification of Polyethylene Glycols (PEGs)-assimilable Bacteria and Culture Characteristics of PEG 6000 Degradation by a Mixed Culture : Micro Degradation of Synthetic Polymers (III) See Reference 1).

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Typical strains of polyethylene glycols (PEGs)-assimilable groups were identified : a PEG 400-assimilable strain, Pseudomonas species; a PEG 600-assimilable strain, Pseudomonas species; a PEG 1000-assimilable strain, Pseudomonas species; a PEG 4000-assimilable strain, Flavobacterium species; a PEG 6000-assimilable pair, Flavobacterium and Pseudomonas species. Culture conditions for PEG 6000 degradation were studied with the mixed culture of Flavobacterium and Pseudomonas species. The optimal culture medium was established to be as follows; PEG 6000,0.5%; (NH_4)HPO_4,0.3%; K_3HPO_4,0.2%; NaH_3PO_4,0.1%; yeast extract, 0.05%; and MgSO_4・7H_2O, 0.05%; in tap water (pH 7.2). cultivation was efficiently carried out at 28℃ for 5-7 days under reciprocal shaking. PEG 6000 in the medium of a 0.5% PEG concentration was almost completely consumed after 7-day culture. During the assimilation of PEG 6000,the viable cell number of Flavobacterium species was dominant and increased with the total growth, while that of Pseudomonas species showed a nearly constant value. A mixture of various PEGs (400,600,1000,1540,2000,4000 and 6000; each 0.1%) was completely degraded by the mixed culture during a 4-day culture. PEG-containing surfactants were also assimilated by PEG-assimilable strains.
社団法人日本生物工学会の論文
1977-10-25