Aerobic Degradation of Polypropylene Glycol by Corynebacterium sp.

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The aerobic metabolism of polypropylene glycol (PPG) was investigated with a strain, Corynebacterium sp. no. 7,which can use PPG as a sole carbon and energy source. PPG was metabolized by washed, resting cells, but it was not degraded by a culture filtrate of the organism or by the cell-free extract. The matabolic products by intact cells were studied using dipropylene glycol (DPG) as a model compund. Three structural isomers of DPG, HOCH(CH_3)CH_3-O-CH_2CH(CH_3)OH, HOCH(CH_3)CH_2-O-CH(CH_3)CH_2OH, and HOCH_2-CH(CH_3)-O-CH(CH_3)CH_2OH, which were sparated and determined by capiillary gas chromatography-mass spectrometry (GC-MS), were degraded. The metabolic products from DPG by intact cells were isolated and identified by GC-MS analysis as OC(CH_3)CH_2-O-CH_2CH(CH_3)OH, OC(CH_3)CH_2-O-CH(CH_3)CH_2OH, and 1,2propanediol plus a small amount of OC(CH_3)CH_2-O-CH_2CO(CH_3). On the other hand, PPG dehydrogenase activity linked with 2,6-dichlorophenolindophenol and phenazine methosulfate was found in sonic extracts of intact cells. The analytical and enzymatic data suggest that PPG is metabolized not by a hydrolytic reaction, but by an oxidative reaction.
1985-06-25