Generation of L7-tTA Knock-in Mice

概要

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We generated a versatile mouse line, L7-tTA knock-in mouse, in whichtetracycline-responsive transcriptional activator (tTA) gene was introduced into exon 2of L7 locus. Since L7 is specifically expressed in cerebellar Purkinje cells, we expectedPurkinje cell-restricted expression of tTA gene in the knock-in mice. In situhybridization analysis exhibited that tTA mRNAs in those mice were expressed only inPurkinje cells. Introduction of transgene consisting of tetracycline-responsive elementthat is a binding site for tTA and ideal cDNAs into L7-tTA knock-in mouse wouldresult in specific expression of cDNA encoding proteins in Purkinje cells and itsexpression could be controlled by doxycycline administration.L7-tTA knock-in mice would provide us with opportunity to elucidate the role ofspecific genes in cerebellar Purkinje cells.