Generation of RGS8 Null Mutant Mice by Cre/loxP System

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概要

• 論文の詳細を見る

• Regulators of G-protein signaling (RGS) proteins are negative regulators ofheterotrimeric guanine-nucleotide-binding proteins (G-proteins) by activating intrinsicGTPase activity of Gα and thereby terminating G-protein coupled receptor-associatedsignal transduction. RGS8 belongs to B/R4 subfamily of RGS proteins and is expressedin the central nervous system, especially dense in cerebellar Purkinje cells. RGS8 bindsspecifically to Gαo and Gαi3 in vitro and activates their intrinsic GTPase activities. Toinvestigate the role of RGS8 in vivo, we generated mice lacking RGS8 by gene targetingin embryonic stem (ES) cells using Cre/loxP system. RGS8 null mutant mice wereviable, fertile and showed apparently normal development. Histological analysisshowed no apparent abnormalities in morphology of cerebellar layer or Purkinje cellsin RGS8 null mutant mice.

• 神戸大学の論文

著者

• Harada Takeshi

  Division of Molecular Genetics, Graduate School of Medicine, Kobe Univ

• Aiba Atsu

  Kobe University Grad. Sch. Medicine

• Aiba Atsu

  Center For Experimental Medicine Institute Of Medical Science University Of Tokyo

• Aiba Atsu

  Division Of Cell Biology Department Of Molecular And Cellular Biology Kobe University Graduate Schoo

• Aiba Atsu

  Division Of Molecular Genetics Department Of Physiology And Cell Biology Kobe University Graduate Sc

• Nakao Kazuki

  Center For Developmental Biology Riken

• Nakao Kazuki

  Laboratory For Animal Resource And Genetic Engineering Riken Center For Developmental Biology

• Nakao Kazuki

  Laboratory For Animal Resources And Genetic Engineering Riken Center For Developmental Biology

• Aiba Atsu

  Division Of Molecular Genetics Graduate School Of Medicine Kobe Univ

• Matsuda Ikuo

  Division Of Molecular Genetics Department Of Physiology And Cell Biology Kobe University Graduate Sc

• Kuwata Hitoshi

  Division of Molecular Genetics, Department of Physiology and Cell Biology, Kobe University Graduate

• Harada Takeshi

  Division Of Molecular Genetics Graduate School Of Medicine Kobe Univ

• Kuwata Hitoshi

  Division Of Molecular Genetics Department Of Physiology And Cell Biology Kobe University Graduate Sc

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