A Novel Selective Nucleoside Phosphorylating Enzyme from Morganella morganii

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概要

• 論文の詳細を見る

• A selective nucleoside phosphorylating enzyme was purified to homogeneity from Morganella morganii NCIMB10466 crude extract. The enzyme appeared to consist of six subunits identical in molecular mass(M_r 25,000). It phosphorylated various nucleosides at the 5'-position to produce nucleoside-5'-monophosphates using pyrophosphate as the phosphate source. Energy-rich compounds, such as carbamylphosphate and acetylphosphate, were also very effective phosphate donors. The enzyme also exhibited phosphatase activity, and dephosphorylated various phosphate esters, but had a weak effect on nucleoside-3'-monophosphates. Based on the results of the kinetic study, the enzyme appeared to be an acid phosphatase. Its activity was partly inhibited by sulfhydryl reagents and heavy metal ions, but not by chelating reagents such as EDTA. Using the purified enzyme, 32.6 mM 5'-IMP was synthesized from inosine with a 41% molar yield, but the synthesized 5'-IMP was hydrolyzed back to inosine and phosphate as the reaction time was extended.

• 社団法人日本生物工学会の論文
• 1999-06-25

著者

• Asano Yasuhisa

  Biotechnology Research Center, Toyama Prefectural University

• Asano Y

  Department Of Immunology And Host Defenses Ehime University Graduate School Of Medicine

• Yamada H

  Department Of Agricultural Chemistry Facutly Of Agriculture Kyoto University

• Yamada H

  Department Of Biotechnology Faculty Of Engineering Tottori University

• Asano Y

  Toyama Prefectural Univ. Toyama Jpn

• Yamada H

  Res. Inst. Tuberculosis Tokyo Jpn

• Yamada Hideaki

  Biotechnology Research Center, Toyama Prefectural University

• Yamada H

  Department Of Agricultural Chemistry Kyoto University

• MIHARA Yasuhiro

  Applied Microbiology Laboratory, Fermentation and Biotechnology Laboratories, Ajinomoto Co., Inc.

• Mihara Y

  Fermentation And Biotechnology Laboratories Ajinomoto Co. Inc.

• Asano Yasuhisa

  Biotechnology Res. Center And Dep. Of Biotechnology Toyama Prefectural Univ.

• Yamada Hideaki

  Biotechnology Research Center Faculty Of Engineering Toyama Prefectural University

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